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Relion 5.0.0 --tomo Refine3D does not use helical parameters from gui input #1281

@MollyGravett

Description

@MollyGravett

Description:
Refine3D does not use helical angular search range or keep tilt prior fixed when input in gui (but Class3D does).

Image

Environment:

  • OS: AlmaLinux 9.4 (Seafoam Ocelot)
  • MPI runtime: Open MPI 4.1.1
  • RELION-5.0.0-commit-adfec8 --tomo

Job options:

  • Type of job: Refine3D
    which relion_refine_mpi --o Refine3D/job045/run --auto_refine --split_random_halves --ios Extract/job004/optimisation_set.star --ref 13pf_apix8.84_box72_andressa.mrc --firstiter_cc --trust_ref_size --ini_high 15 --dont_combine_weights_via_disc --scratch_dir /scratch/molly3 --pool 10 --pad 2 --ctf --ctf_intact_first_peak --particle_diameter 570 --flatten_solvent --zero_mask --solvent_mask MaskCreate/job018/mask.mrc --oversampling 1 --healpix_order 4 --auto_local_healpix_order 4 --offset_range 4 --offset_step 1 --sym C1 --low_resol_join_halves 40 --norm --scale --helix --helical_inner_diameter 110 --helical_outer_diameter 390 --ignore_helical_symmetry --helical_keep_tilt_prior_fixed --j 2 --gpu "" --pipeline_control Refine3D/job045/
  • Type of job: Class3D
    which relion_refine_mpi --o Class3D/job045/run --ios Extract/job004/optimisation_set.star --ref 13pf_apix8.84_box72_andressa.mrc --firstiter_cc --trust_ref_size --ini_high 20 --dont_combine_weights_via_disc --scratch_dir /scratch/molly --pool 10 --pad 2 --iter 1 --tau2_fudge 1 --particle_diameter 570 --K 1 --flatten_solvent --zero_mask --oversampling 1 --healpix_order 2 --offset_range 4 --offset_step 1 --sym C1 --norm --scale --helix --helical_inner_diameter 110 --helical_outer_diameter 390 --ignore_helical_symmetry --helical_keep_tilt_prior_fixed --sigma_tilt 5 --sigma_psi 3.33333 --sigma_rot 0 --j 4 --gpu "" --pipeline_control Class3D/job045/

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