diff --git a/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.hash b/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.hash
index 6f77b1bfd0..e8f9ad0a48 100644
--- a/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.hash
+++ b/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.hash
@@ -1,2 +1,2 @@
 # locally computed
-sha256 667860ca6dbfd59849de001007e7c11b95732746c736528a7641bafdff7b636e  thermocycler-gen2@v1.0.0.bin
+sha256 9a4043d9790926fbb3b5a8b760b2be5e6918e38f94a2a595e11d50ea1e16ad37  thermocycler-gen2@v1.0.1.bin
diff --git a/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.mk b/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.mk
index b995fc548e..2436eb3005 100644
--- a/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.mk
+++ b/package/opentrons/opentrons-thermocycler-gen2-firmware/opentrons-thermocycler-gen2-firmware.mk
@@ -4,8 +4,8 @@
 #
 ################################################################################
 
-OPENTRONS_THERMOCYCLER_GEN2_FIRMWARE_VERSION=v1.0.0
-OPENTRONS_THERMOCYCLER_GEN2_FIRMWARE_SOURCE=thermocycler-gen2@v1.0.0.bin
+OPENTRONS_THERMOCYCLER_GEN2_FIRMWARE_VERSION=v1.0.1
+OPENTRONS_THERMOCYCLER_GEN2_FIRMWARE_SOURCE=thermocycler-gen2@v1.0.1.bin
 OPENTRONS_THERMOCYCLER_GEN2_FIRMWARE_SITE=https://opentrons-modules-builds.s3.us-east-2.amazonaws.com/thermocycler-gen2/$(OPENTRONS_THERMOCYCLER_GEN2_FIRMWARE_VERSION)
 OPENTRONS_THERMOCYCLER_GEN2_FIRMWARE_LICENSE=Apache-2.0