diff --git a/CHANGELOG.md b/CHANGELOG.md
index 436dd6abc..fb6edc3f9 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -20,6 +20,7 @@ Write the date in place of the "Unreleased" in the case a new version is release
   documentation and one test.
 - Deletion of nodes or metadata revisions now requires deletion scopes,
   rather than writing scopes.
+- In-memory SQLite databases are connection pooled / cached.
 
 ## Fixed
 
diff --git a/tiled/_tests/test_sync.py b/tiled/_tests/test_sync.py
index a2fc2131a..789e48f50 100644
--- a/tiled/_tests/test_sync.py
+++ b/tiled/_tests/test_sync.py
@@ -1,6 +1,7 @@
 import asyncio
 import contextlib
 import tempfile
+import uuid
 
 import awkward
 import h5py
@@ -24,7 +25,9 @@
 def client_factory(readable_storage=None):
     with tempfile.TemporaryDirectory() as tempdir:
         catalog = in_memory(
-            writable_storage=str(tempdir), readable_storage=readable_storage
+            named_memory=str(uuid.uuid4())[:8],
+            writable_storage=str(tempdir),
+            readable_storage=readable_storage,
         )
         app = build_app(catalog)
         with Context.from_app(app) as context:
diff --git a/tiled/_tests/test_validation.py b/tiled/_tests/test_validation.py
index b58089e0f..ec397b758 100644
--- a/tiled/_tests/test_validation.py
+++ b/tiled/_tests/test_validation.py
@@ -1,6 +1,8 @@
 """
 This tests tiled's validation registry
 """
+import uuid
+
 import numpy as np
 import pandas as pd
 import pytest
@@ -50,7 +52,10 @@ def client(tmpdir_module):
             {
                 "tree": "tiled.catalog:in_memory",
                 "path": "/",
-                "args": {"writable_storage": str(tmpdir_module)},
+                "args": {
+                    "named_memory": str(uuid.uuid4())[:8],
+                    "writable_storage": str(tmpdir_module),
+                },
             },
         ],
         "specs": [
@@ -104,7 +109,10 @@ def test_unknown_spec_strict(tmpdir):
             {
                 "tree": "tiled.catalog:in_memory",
                 "path": "/",
-                "args": {"writable_storage": str(tmpdir)},
+                "args": {
+                    "named_memory": str(uuid.uuid4())[:8],
+                    "writable_storage": str(tmpdir),
+                },
             },
         ],
         "specs": [
diff --git a/tiled/server/app.py b/tiled/server/app.py
index b8a4cfed3..0a10cab8a 100644
--- a/tiled/server/app.py
+++ b/tiled/server/app.py
@@ -562,13 +562,13 @@ async def startup_event():
                 make_admin_by_identity,
                 purge_expired,
             )
-            from .connection_pool import open_database_connection_pool
+            from .connection_pool import is_memory_sqlite, open_database_connection_pool
 
             # This creates a connection pool and stashes it in a module-global
             # registry, keyed on database_settings, where it can be retrieved by
             # the Dependency get_database_session.
             engine = open_database_connection_pool(settings.database_settings)
-            if not engine.url.database or engine.url.query.get("mode") == "memory":
+            if is_memory_sqlite(engine.url):
                 # Special-case for in-memory SQLite: Because it is transient we can
                 # skip over anything related to migrations.
                 await initialize_database(engine)
diff --git a/tiled/server/connection_pool.py b/tiled/server/connection_pool.py
index 60d066348..6bf4ed614 100644
--- a/tiled/server/connection_pool.py
+++ b/tiled/server/connection_pool.py
@@ -7,7 +7,7 @@
 from sqlalchemy import event
 from sqlalchemy.engine import URL, make_url
 from sqlalchemy.ext.asyncio import AsyncEngine, AsyncSession, create_async_engine
-from sqlalchemy.pool import AsyncAdaptedQueuePool
+from sqlalchemy.pool import AsyncAdaptedQueuePool, StaticPool
 
 from ..server.settings import DatabaseSettings, Settings, get_settings
 from ..utils import ensure_specified_sql_driver, safe_json_dump, sanitize_uri
@@ -60,8 +60,8 @@ def open_database_connection_pool(database_settings: DatabaseSettings) -> AsyncE
             ensure_specified_sql_driver(database_settings.uri),
             echo=DEFAULT_ECHO,
             json_serializer=json_serializer,
+            poolclass=StaticPool,
         )
-
     else:
         engine = create_async_engine(
             ensure_specified_sql_driver(database_settings.uri),
@@ -73,9 +73,9 @@ def open_database_connection_pool(database_settings: DatabaseSettings) -> AsyncE
             pool_pre_ping=database_settings.pool_pre_ping,
         )
 
-        # Cache the engine so we don't create more than one pool per database_settings.
-        monitor_db_pool(engine.pool, sanitize_uri(database_settings.uri)[0])
-        _connection_pools[database_settings] = engine
+    # Cache the engine so we don't create more than one pool per database_settings.
+    monitor_db_pool(engine.pool, sanitize_uri(database_settings.uri)[0])
+    _connection_pools[database_settings] = engine
 
     # For SQLite, ensure that foreign key constraints are enforced.
     if engine.dialect.name == "sqlite":