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## Ribosome Binding Site (RBS) Motifs
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This document provides information on Ribosome Binding Site (RBS) motifs based on the Prodigal paper. The RBS motifs are categorized by spacer ranges, which indicate the number of base pairs (bp) between the RBS and the start codon.
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This document provides an overview of Ribosome Binding Site (RBS) motifs, as described in the Prodigal paper. RBS motifs are categorized based on the spacer range, which represents the number of base pairs (bp) between the RBS and the start codon.
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### Spacer Ranges and Representative RBS Motifs
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Example sequence with RBS motif:
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The table below lists the spacer ranges and their corresponding representative RBS motifs:
Three different window checking ranges are defined to identify potential RBS motifs relative to the start codon (ATG):
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To identify potential RBS motifs relative to the start codon (ATG), three window checking ranges are defined:
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- Window Checking A:
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```
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-10 -3 |-> start codon
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...|......|..ATG...
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```
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-**Window Checking A**:
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```
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-10 -3 |-> start codon
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...|......|..ATG...
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```
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- Window Checking B:
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```
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-16 -5 |-> start codon
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...|..........|....ATG...
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```
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-**Window Checking B**:
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```
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-16 -5 |-> start codon
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...|..........|....ATG...
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```
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- Window Checking C:
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```
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-20 -11 |-> start codon
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.|........|..........ATG...
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```
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-**Window Checking C**:
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```
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-20 -11 |-> start codon
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.|........|..........ATG...
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```
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### RBS Motif Scoring
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The RBS motif scoring is based on the Prodigal paper, which uses a scoring system to identify potential RBS motifs. The scoring system is as follows:
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The scoring of RBS motifs is based on the Prodigal paper. This scoring system helps identify the most likely RBS motifs for a given sequence. The scoring criteria are implemented in the `orf_rbs_score` function (aliased as `orbs`).
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### Example Usage
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### Example
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An example of an exact search query for an RBS motif is provided:
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Below is an example of how to inspect RBS motifs for a given ORF using the `_findrbs` function:
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-**ExactSearchQuery(dna"ATACG", iscompatible)** returns 5, indicating the position of the motif.
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|----|:RBS(dna"GGAGGA", 1:5, :A, 27, STRAND_POS)
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```
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To inspect the RBS motifs of a given ORF, we can use the `_findrbs` function. This function returns the RBS motifs of a given ORF:
Now, following the scoring scheme mentioned above, we can calculate the RBS score for each motif. This criteria is implemented in the `orf_rbs_score` or simply aliased to `orbs` function:
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### Calculating RBS Scores
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Using the `orf_rbs_score` function, you can calculate the RBS score for each motif:
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