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| 1 | +## Finding complete and overlapped ORFIs |
| 2 | + |
| 3 | +The main package function is `findorfs`. Under the hood, the `findorfs` function is an interface for different gene finding algorithms that can be plugged using the `finder` keyword argument. By default it uses the `NaiveFinder` algorithm, which is a simple algorithm that finds all (non-outbounded) ORFIs in a DNA sequence (see the [NaiveFinder](https://camilogarciabotero.github.io/GeneFinder.jl/dev/api/#GeneFinder.NaiveFinder-Union{Tuple{Union{BioSequences.LongDNA{N},%20BioSequences.LongSubSeq{BioSequences.DNAAlphabet{N}}}},%20Tuple{N}}%20where%20N) documentation for more details). |
| 4 | + |
| 5 | +> [!NOTE] |
| 6 | + The `minlen` kwarg in the `NaiveFinder` mehtod has been set to 6nt, so it will catch random ORFIs not necesarily genes thus it might consider `dna"ATGTGA"` -> `aa"M*"` as a plausible ORFI. |
| 7 | +
|
| 8 | +Here is an example of how to use the `findorfs` function with the `NaiveFinder` algorithm: |
| 9 | + |
| 10 | +```julia |
| 11 | +using BioSequences, GeneFinder |
| 12 | + |
| 13 | +# > 180195.SAMN03785337.LFLS01000089 -> finds only 1 gene in Prodigal (from Pyrodigal tests) |
| 14 | +seq = dna"AACCAGGGCAATATCAGTACCGCGGGCAATGCAACCCTGACTGCCGGCGGTAACCTGAACAGCACTGGCAATCTGACTGTGGGCGGTGTTACCAACGGCACTGCTACTACTGGCAACATCGCACTGACCGGTAACAATGCGCTGAGCGGTCCGGTCAATCTGAATGCGTCGAATGGCACGGTGACCTTGAACACGACCGGCAATACCACGCTCGGTAACGTGACGGCACAAGGCAATGTGACGACCAATGTGTCCAACGGCAGTCTGACGGTTACCGGCAATACGACAGGTGCCAACACCAACCTCAGTGCCAGCGGCAACCTGACCGTGGGTAACCAGGGCAATATCAGTACCGCAGGCAATGCAACCCTGACGGCCGGCGACAACCTGACGAGCACTGGCAATCTGACTGTGGGCGGCGTCACCAACGGCACGGCCACCACCGGCAACATCGCGCTGACCGGTAACAATGCACTGGCTGGTCCTGTCAATCTGAACGCGCCGAACGGCACCGTGACCCTGAACACAACCGGCAATACCACGCTGGGTAATGTCACCGCACAAGGCAATGTGACGACTAATGTGTCCAACGGCAGCCTGACAGTCGCTGGCAATACCACAGGTGCCAACACCAACCTGAGTGCCAGCGGCAATCTGACCGTGGGCAACCAGGGCAATATCAGTACCGCGGGCAATGCAACCCTGACTGCCGGCGGTAACCTGAGC" |
| 15 | + |
| 16 | +orfs = findorfs(seq, finder=NaiveFinder) # use finder=NaiveCollector as an alternative |
| 17 | + |
| 18 | +12-element Vector{ORFI{4, NaiveFinder}}: |
| 19 | + ORFI{NaiveFinder}(29:40, '+', 2) |
| 20 | + ORFI{NaiveFinder}(137:145, '+', 2) |
| 21 | + ORFI{NaiveFinder}(164:184, '+', 2) |
| 22 | + ORFI{NaiveFinder}(173:184, '+', 2) |
| 23 | + ORFI{NaiveFinder}(236:241, '+', 2) |
| 24 | + ORFI{NaiveFinder}(248:268, '+', 2) |
| 25 | + ORFI{NaiveFinder}(362:373, '+', 2) |
| 26 | + ORFI{NaiveFinder}(470:496, '+', 2) |
| 27 | + ORFI{NaiveFinder}(551:574, '+', 2) |
| 28 | + ORFI{NaiveFinder}(569:574, '+', 2) |
| 29 | + ORFI{NaiveFinder}(581:601, '+', 2) |
| 30 | + ORFI{NaiveFinder}(695:706, '+', 2) |
| 31 | +``` |
| 32 | + |
| 33 | +The `ORFI` structure displays the location, frame, and strand, but currently does not include the sequence *per se*. To extract the sequence of an `ORFI` instance, you can use the `sequence` method directly on it, or you can also broadcast it over the `orfs` collection using the dot syntax `.`: |
| 34 | + |
| 35 | +```julia |
| 36 | +sequence.(orfs) |
| 37 | + |
| 38 | +12-element Vector{LongSubSeq{DNAAlphabet{4}}}: |
| 39 | + ATGCAACCCTGA |
| 40 | + ATGCGCTGA |
| 41 | + ATGCGTCGAATGGCACGGTGA |
| 42 | + ATGGCACGGTGA |
| 43 | + ATGTGA |
| 44 | + ATGTGTCCAACGGCAGTCTGA |
| 45 | + ATGCAACCCTGA |
| 46 | + ATGCACTGGCTGGTCCTGTCAATCTGA |
| 47 | + ATGTCACCGCACAAGGCAATGTGA |
| 48 | + ATGTGA |
| 49 | + ATGTGTCCAACGGCAGCCTGA |
| 50 | + ATGCAACCCTGA |
| 51 | +``` |
| 52 | + |
| 53 | +Similarly, you can extract the amino acid sequences of the ORFIs using the `translate` function. |
| 54 | + |
| 55 | +```julia |
| 56 | +translate.(orfs) |
| 57 | + |
| 58 | +12-element Vector{LongAA}: |
| 59 | + MQP* |
| 60 | + MR* |
| 61 | + MRRMAR* |
| 62 | + MAR* |
| 63 | + M* |
| 64 | + MCPTAV* |
| 65 | + MQP* |
| 66 | + MHWLVLSI* |
| 67 | + MSPHKAM* |
| 68 | + M* |
| 69 | + MCPTAA* |
| 70 | + MQP* |
| 71 | +``` |
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