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Description
When a fastq has a missing 1st entry (due to short reads and trimming) seqkit seq infers that the type of sequences in the file is "unlimit" rather than "dna": https://bioinf.shenwei.me/seqkit/usage/#seq
"unlimit" type sequences cannot be complemented and the files currently returned by --read_transform "reverse_complement" are just reversed. This will mean no reads match template libraries for quantification.
Specify -t dna or --seq-type dna will fix. This could limits quants from working with RNA sequences - is this a potential issue?