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I am facing a platform that output Barcode in reverse-complemented form with Illumina sequencing primers. (the barcode will be normal with their own primer which is on the other side)
It will be helpful if fgbio can reverse-complement a read structure, for example, when the type is in lower case.
Will you consider make '30b+S10M' to work as the first 30 bp need to be reverse-complemented and then be used as barcode, and the last 10 bp to directly used as UMI ?
The text was updated successfully, but these errors were encountered:
@galaxy001 I can empathize with the desire to have this support in the tools. I assume you're going to demultiplex your data with fqtk demux or the like, in which case you'll be supplying the sample metadata including the expected sample barcode sequences. In that case, I would recommend that they be provided in sequencing order (i.e. reverse complement them in the sample metadata/sample sheet).
I am facing a platform that output Barcode in reverse-complemented form with Illumina sequencing primers. (the barcode will be normal with their own primer which is on the other side)
It will be helpful if
fgbio
can reverse-complement a read structure, for example, when the type is in lower case.Will you consider make '30b+S10M' to work as the first 30 bp need to be reverse-complemented and then be used as barcode, and the last 10 bp to directly used as UMI ?
The text was updated successfully, but these errors were encountered: