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Currently, SetMateInformation does not add the MQ tag to supplementary alignments.
I'm not sure if this is intended behavior - the docs suggest that supplementary alignments would have this tag added.
Adds and/or fixes mate information on paired-end reads. Sets the MQ (mate mapping quality), 'MC' (mate cigar string),
ensures all mate-related flag fields are set correctly, and that the mate reference and mate start position are
correct.
Supplementary records are handled correctly (updated with their mate's non-supplemental attributes). Secondary
alignments are passed through but are not updated.
GroupReadsByUmi requires that all alignments, including supplementary alignments, have the MQ tag set, so it would be helpful if SetMateInformation produced a compatible BAM 🙂
The text was updated successfully, but these errors were encountered:
It looks like MQ is not set on the supplementary records, so we'd likely need to make a PR into htsjdk, wait for a release, then update fgbio's htsjdk dependency.
Currently,
SetMateInformation
does not add theMQ
tag to supplementary alignments.I'm not sure if this is intended behavior - the docs suggest that supplementary alignments would have this tag added.
GroupReadsByUmi
requires that all alignments, including supplementary alignments, have theMQ
tag set, so it would be helpful ifSetMateInformation
produced a compatible BAM 🙂The text was updated successfully, but these errors were encountered: