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gofrFromCoords.m
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gofrFromCoords.m
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% Get all coordinate files
MainFolder = 'C:\Users\gdbenn\Documents\Data\Georginadata\WholeCellData\deltaOmpR+OmpR_Supressed\out\';
FilePattern = strcat(MainFolder, '*.csv');
Files = dir(FilePattern);
% Set bin parameters
BinSize = 15;
Edges = 0:BinSize:2500;
Middles = (BinSize/2):BinSize:(2500-(BinSize/2));
% For each sample
for k = 1 : length(Files)
% Get real cooridinates file
CoordsFile = strcat(Files(k).folder, '\', Files(k).name);
RealCoordsTable = readtable(CoordsFile);
Coords = table2array(RealCoordsTable(:,:));
% Convert coordinates from pixels to nm
Coords = Coords*512/500;
% Get all distances for the real data
AllRealDists = [];
% Take each coordinate at a time
for i = 1 : length(Coords)
DistsForGR = [];
% Get the distance between coordinate i and all other coordinates
for j = 1 : length(Coords)
Dist = sqrt((Coords(i,1)- Coords(j,1))^2 + (Coords(i,2)- Coords(j,2))^2);
% Add all the distances in one column
DistsForGR = [DistsForGR;Dist];
end
% For g(r) append all distances between all coordinates together in
% one enormous array
AllRealDists = [AllRealDists,DistsForGR];
end
% Remove repeats from distances as each pair is compared twice
SizeDists = size(AllRealDists);
for i = 1 : SizeDists(1)
AllRealDists(i:end,i) = NaN;
end
% Save real distances
DistsFilename = strcat(Files(k).folder, '\Out\', strrep(Files(k).name, '.csv', 'all_dists_real.txt'));
writematrix(AllRealDists, DistsFilename);
% Put all distances in one column
RealDists1D = AllRealDists(:);
% Remove NaNs and sort lowest to highest
RealDists1D = sort(RealDists1D(~isnan(RealDists1D)));
% Take each RandomCoords file for this sample and get distances for gr
FileParts = split(Files(k).name, 'CLAHE');
AllRanNNs = [];
AllRanDists1D = [];
% For all 5 random coordinate files...
for n = 1 : 5
if n == 1
RanCoordsFile = strcat(Files(k).folder, '\Out\', FileParts{1}, 'RanCoords1', '.csv');
else
RanCoordsFile = strcat(Files(k).folder, '\Out\', FileParts{1}, 'RanCoords', num2str(n), '.csv');
end
% ... open coordinates
RanCoordsTable = readtable(RanCoordsFile);
RanCoords = table2array(RanCoordsTable);
% Take each coord at a time
AllRanDists = [];
for i = 1 : length(RanCoords)
DistsForGR = [];
% Get the distance between coordinate i and all other coordinates
for j = 1 : length(RanCoords)
Dist = sqrt((RanCoords(i,1)- RanCoords(j,1))^2 + (RanCoords(i,2)- RanCoords(j,2))^2);
% Add all the distances in one column
DistsForGR = [DistsForGR;Dist];
end
% For g(r) append all distances together in one enormous array
AllRanDists = [AllRanDists,DistsForGR];
end
% Save random distances
ranDistsFilename = strcat(Files(k).folder, '\Out\', strrep(Files(k).name, '.csv', 'all_dists'), '_Ran', num2str(n), '.txt');
writematrix(AllRanDists, ranDistsFilename);
% Remove repeats
SizeDists = size(AllRanDists);
for i = 1 : SizeDists(1)
AllRanDists(i:end,i) = NaN;
end
% Put all dists in one column
RanDists1D = AllRanDists(:);
% Remove NaNs and sort lowest to highest
RanDists1D = sort(RanDists1D(~isnan(RanDists1D)));
% Put all Ran distances in one array
AllRanDists1D = [AllRanDists1D,RanDists1D];
end
AllDists = [RealDists1D,AllRanDists1D];
% Get g(r)
% Get area used by opening area files, converting to binary and summing
% pixels
AreaFile = strcat(strrep(Files(k).folder, '\out', '\'), strrep(Files(k).name, 'CLAHE_prediction.csv', 'area.tiff'));
AreaNumber = imread(AreaFile);
Area = 500*(sum(AreaNumber,'all'))/(255*512);
% Bin all distances to save
AllBinned = Middles.';
for n = 1 : 6
N = (histcounts(AllDists(:,n),Edges)).';
AllBinned = [AllBinned,N];
end
% Save binned distances
BinnedDistFilename = strcat(Files(k).folder, '\Out\', strrep(Files(k).name, '.csv', 'binned_dists.txt'));
writematrix(AllBinned, BinnedDistFilename);
NumDists = sum(AllBinned(:,2));
BinnedNoMiddles = AllBinned(:,2:end);
gr = [];
% For real and all random cases
for i = 1 : size(BinnedNoMiddles,1)
grcol = [];
% Take each bin
for j = 1 : size(BinnedNoMiddles,2)
% Calculate g(r)
grij = (BinnedNoMiddles(i,j)*Area)/(2*pi*Middles(i)*BinSize*NumDists);
% Append for each bin
grcol = [grcol,grij];
end
% Append for each sample
gr = [gr;grcol];
end
grwithMiddles = [Middles.',gr];
% Save grs
grFilename = strcat(Files(k).folder, '\Out\', strrep(Files(k).name, '.csv', 'grs.txt'));
writematrix(grwithMiddles, grFilename);
end