8-bit ND2 files stretch bug

[Simone-Massalini]

Some ND2 files (acquired with NIS elements software, Nikon) in 8-bit at certain resolutions (e.g. 1066x590, 1277x1021) look stretched when opened in Fiji (attached image – left opened in Fiji, right the same image in NIS-Elements).
The files contain multiple time points (fast timelapses).

[ctrueden]

@Simone-Massalini ImageJ does not have built-in support for ND2. How are you opening your ND2 files in Fiji? Via Bio-Formats?

[Simone-Massalini]

@ctrueden I am just using Fiji as it is, hyperstack and no other option selected. It works pretty well with 3200x3200 images (16-bit).

[ctrueden]

@Simone-Massalini OK, thanks, that means Bio-Formats is being used under the hood. The best thing to do is report the issue on the Image.sc Forum as described in the Bio-Formats bug reporting guidelines. And it will be super helpful if you can include a sample ND2 file exhibiting the issue!