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Move TrackMate-Weka images in the detectors subfolder.
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_pages/plugins/trackmate/detectors/trackmate-weka.md

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@@ -11,7 +11,7 @@ This page describes a detector module for [TrackMate](/plugins/trackmate/index)
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For this module to work, you just need to install the TrackMate module. Subscribe to the **TrackMate-Weka** update site.
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{% include img src='/media/plugins/trackmate/trackmate-weka-install.png' align='center' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-install.png' align='center' %}
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## Tutorial: tracking focal adhesions
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The movie included show human dermal microvascular blood endothelial cells expressing Paxillin, imaged with a spinning-disk confocal microscope.
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image8.png' align='center' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image8.png' align='center' %}
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The goal is to track the focal adhesions staying at the cell periphery.
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They are in general brighter than the cell body and the image background, but with a high variance in their intensities.
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The figure below illustrates the Weka GUI with two classes: one in red for focal adhesions and one in green for the others (cell body, image background).
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More details on training the classifier can be found from on the [plugin documentation page](/plugins/tws).
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image5.png' align='center' width='400' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image5.png' align='center' width='400' %}
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### Using Weka in TrackMate
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Now open the image in Fiji and launch TrackMate, click Next and select Weka detector from the dropdown menu
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image4.png' align='center' width='250' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image4.png' align='center' width='250' %}
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In the next panel, browse the classifier file, choose the target class as `FocalAdhesion` and set the threshold probability as 0.5.
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image7.png' align='center' width='250' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image7.png' align='center' width='250' %}
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Click on `Preview` button to check the segmentation result, then click Next to run the segmentation for all time frames. This step takes about 8 minutes on a standard pc.
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image2.png' align='center' width='400' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image2.png' align='center' width='400' %}
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Once the detection process is done, click `Next` until the step `Select a tracker`, here we choose the `Simple LAP tracker` and set the `Linking max distance` and the `Gap-closing max distance` are both equal 2.0 microns, the `Gap-closing max frame gap` is set as 2.
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Click `Next` and wait for the tracking process to finish.
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image1.png' align='center' width='250' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image1.png' align='center' width='250' %}
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One purpose of tracking the focal adhesions is to study how they shrink or expand over time. TrackMate provides an option to color the tracks by time. Click Next until the step Display options and select Frame in Color tracks by, then click auto.
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image3.png' align='center' width='250' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image3.png' align='center' width='250' %}
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Looking at the tracking result shown in the movie below, the focal adhesions localizing at the top are shrinking whereas the ones at the bottom right are expanding.
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{% include img src='/media/plugins/trackmate/trackmate-weka-detector-image6.gif' align='center' %}
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{% include img src='/media/plugins/trackmate/detectorstrackmate-weka-detector-image6.gif' align='center' %}
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__________________
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Minh-Son Phan, August 2021

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