-
Notifications
You must be signed in to change notification settings - Fork 4
mesoSPIM_immersion_cuvettes
Fabian F. Voigt edited this page Mar 11, 2019
·
2 revisions
Immersion cuvettes holders are based on X-95 carriers, a tip-tilt plate
to adjust them relative to the light-sheet, and the immersion cuvette itself.
They are designed to fit all possible XYZ and rotation positions of the
sample.
In order to attach the kinematic base to the tip-tilt plates, the central
M4 screw of the tip-tilt plate has to be removed.
-
Background
- mesoSPIM history
- Optical design
- Electronics
-
Setting up a mesoSPIM
- First steps
- Preparing the software and electronics
- Preparing the microscope optics
-
Setting the microscope up
- General alignment tips and tricks
- Installing the microscope base
- Setting up the detection path
- Alignment of the detection path
- Setup of the sample XYZ stages
- Setup of the excitation path
- Immersion cuvettes
- Set up a microscope config file
- Light-sheet co-alignment
- Set up initial ETL parameters
- Setting up lasers with the GUI
- Sample Handling
- Test Samples
- Troubleshooting
- Upgrades and custom variants