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CHANGELOG.md

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The format is based on [Keep a Changelog](http://keepachangelog.com/)
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and this project adheres to [Semantic Versioning](http://semver.org/).
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## Unpublished
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## [v2.2] - 2024-09-26
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### Changes
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README.md

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quicksand is executed directly from github, no local build is required. With the databases and the testdata downloaded, run the pipeline.
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```bash
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nextflow run mpieva/quicksand -r v2.1 \
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nextflow run mpieva/quicksand -r v2.2 \
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--db refseq/kraken/Mito_db_kmer22/ \
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--genomes refseq/genomes/ \
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--bedfiles refseq/masked/ \

assets/pipeline/help.txt

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Usage: nextflow run mpieva/quicksand -r v2.1 {--bam PATH --rg PATH | --split DIR} --db PATH --genome PATH --bedfiles PATH [options] -profile singularity
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Usage: nextflow run mpieva/quicksand -r v2.2 {--bam PATH --rg PATH | --split DIR} --db PATH --genome PATH --bedfiles PATH [options] -profile singularity
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required arguments:
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Either:

docs/source/conf.py

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author = 'Merlin Szymanski'
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# The full version, including alpha/beta/rc tags
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release = 'v2.1'
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version = 'v2.1'
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release = 'v2.2'
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version = 'v2.2'
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# -- General configuration ---------------------------------------------------
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docs/source/examples.rst

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Execute quicksand like this::
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nextflow run mpieva/quicksand -r v2.1 \
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nextflow run mpieva/quicksand -r v2.2 \
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-profile singularity \
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--split split/ \
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--db refseq/kraken/Mito_db_kmer22/ \
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after the KrakenUniq run are stored in :code:`out/{family}/1-extracted/` while mapped, deduped and filtered sequences are saved to the
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:code:`out/{family}/best/{step}/` directory after the respective processing step::
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quicksand_v2.1
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quicksand_v2.2
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├── out
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│ └── {family}
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│ ├── 1-extracted
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and start the execution with::
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nextflow run mpieva/quicksand -r v2.1 \
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nextflow run mpieva/quicksand -r v2.2 \
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-profile singularity \
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--split split/ \
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--db refseq/kraken/Mito_db_kmer22/ \
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appear in the :code:`out/{family}/fixed/{step}/` directory, together with additional output-files
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that are useful in additional downstream-analyses, such as the extracted deaminated reads::
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quicksand_v2.1
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quicksand_v2.2
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├── out
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│ └── {family}
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│ ├── 1-extracted
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and rerun the pipeline with::
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nextflow run mpieva/quicksand -r v2.1 \
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nextflow run mpieva/quicksand -r v2.2 \
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-profile singularity \
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--rerun \
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--fixed fixed-references.tsv
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The (additional) output files are the ones created by the :code:`--fixed` flag::
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quicksand_v2.1
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quicksand_v2.2
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├── out
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│ └── {family}
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│ ├── 1-extracted

docs/source/in_and_out.rst

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Output
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------
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quicksand writes all output files to the **quicksand_v2.1** directory. Within this directory the files are
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quicksand writes all output files to the **quicksand_v2.2** directory. Within this directory the files are
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layed out as follows::
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quicksand_v2.1
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quicksand_v2.2
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├── out
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│ └── {taxon}
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│ ├── 1-extracted

docs/source/quickstart.rst

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nextflow pipelines can be executed directly from github. To run quicksand using the downloaded data-set type::
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nextflow run mpieva/quicksand -r v2.1 \
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nextflow run mpieva/quicksand -r v2.2 \
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-profile singularity \
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--db refseq/kraken/Mito_db_kmer22 \
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--genomes refseq/genomes/ \
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--bedfiles refseq/masked/ \
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--split split/
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| The output of quicksand can be found in the directory **quicksand_v2.1/**
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| The output of quicksand can be found in the directory **quicksand_v2.2/**
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| See the :code:`final_report.tsv` and :code:`filtered_report_0.5p_0.5b.tsv` for a summary of the results.
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| See the :ref:`output-page` section for a detailed explaination of all the output files.
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nextflow.config

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homePage = 'https://mpieva.github.io/quicksand/'
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description = 'quick analysis of sedimentary ancient DNA'
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nextflowVersion = '>=22.10'
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version = 'v2.1'
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version = 'v2.2'
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}
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cleanup = true

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