@@ -85,7 +85,7 @@ All files are prefixed (`<prefix>`) as the input genome fasta file.
8585Platon can be installed in 2 different ways, though we advise to use Conda/BioConda.
8686
8787In all cases, the custom database must be downloaded which we provide for download:
88- [ ![ DOI] ( https://zenodo.org/badge/DOI/10.5281/zenodo.3349651 .svg )] ( https://doi.org/10.5281/zenodo.3349651 )
88+ [ ![ DOI] ( https://zenodo.org/badge/DOI/10.5281/zenodo.4066768 .svg )] ( https://doi.org/10.5281/zenodo.4066768 )
8989
9090### BioConda
91911 . install Platon via [ Conda] ( https://conda.io/docs/install/quick.html ) and the [ Bioconda] ( https://bioconda.github.io/ ) channel
@@ -94,7 +94,7 @@ In all cases, the custom database must be downloaded which we provide for downlo
9494Example:
9595```
9696$ conda install -c conda-forge -c bioconda -c defaults platon
97- $ wget https://zenodo.org/record/3924529 /files/db.tar.gz
97+ $ wget https://zenodo.org/record/4066768 /files/db.tar.gz
9898$ tar -xzf db.tar.gz
9999$ rm db.tar.gz
100100$ platon --db ./db genome.fasta
111111$ cd platon
112112$ python3 -m pip install .
113113$ cd ..
114- $ wget https://zenodo.org/record/3924529 /files/db.tar.gz
114+ $ wget https://zenodo.org/record/4066768 /files/db.tar.gz
115115$ tar -xzf db.tar.gz
116116$ rm db.tar.gz
117117$ platon/bin/platon --db ./db genome.fasta
@@ -178,14 +178,14 @@ Platon provides 3 different modi controlling which filters will be used.
178178In the ` sensitivity ` mode Platon will classifiy all contigs with an ` RDS ` value * below*
179179the sensitivity threshold as chromosomal and all remaining contigs as plasmid.
180180This threshold was defined to account for 95% sensitivity and computed via Monte Carlo
181- simulations of artifical contigs resulting in an RDS=-7.7 .
181+ simulations of artifical contigs resulting in an RDS=-7.9 .
182182-> use this mode to * exclude chromosomal* contigs.
183183
184184### Specificity
185185In the ` specificity ` mode Platon will classifiy all contigs with an ` RDS ` value * above*
186186the specificity threshold as plasmid and all remaining contigs as chromosomal.
187187This threshold was defined to account for 99.9% specificity and computed via Monte Carlo
188- simulations of artifical contigs resulting in an RDS=0.4 .
188+ simulations of artifical contigs resulting in an RDS=0.7 .
189189
190190### Accuracy (default)
191191In the ` accuracy ` mode Platon will classifiy all contigs with:
@@ -204,9 +204,9 @@ Platon depends on a custom database based on MPS, RDS, RefSeq Plasmid database,
204204PlasmidFinder db as well as manually curated MOB HMM models from MOBscan,
205205custom conjugation and replication HMM models and oriT sequences from MOB-suite.
206206This database based on UniProt UniRef90 release 2020_01 can be downloaded here:
207- (zipped 1.4 Gb, unzipped 2.4 Gb)
208- [ ![ DOI] ( https://zenodo.org/badge/DOI/10.5281/zenodo.3349651 .svg )] ( https://doi.org/10.5281/zenodo.3349651 )
209- - [ https://zenodo.org/record/3924529 /files/db.tar.gz ] ( https://zenodo.org/record/3924529 /files/db.tar.gz )
207+ (zipped 1.6 Gb, unzipped 2.8 Gb)
208+ [ ![ DOI] ( https://zenodo.org/badge/DOI/10.5281/zenodo.4066768 .svg )] ( https://doi.org/10.5281/zenodo.4066768 )
209+ - [ https://zenodo.org/record/4066768 /files/db.tar.gz ] ( https://zenodo.org/record/4066768 /files/db.tar.gz )
210210
211211## Dependencies
212212Platon was developed and tested in Python 3.5 and depends on BioPython (>=1.71).
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