Testing for interactions #277
Description
I am currently working on DGE (liver; males and females) data from a lab experiment where I have reared stickleback in contrasting temperatures and diets. Is there a way to test for interactions using sleuth? Currently I have tried the following to models to test for the interaction between temperature and sex.
base_model <- temperature + sex+ diet
full_model <- temperature * sex + diet
The result for one of the genes I am interested in (Vtg3) is non-significant, however, when I plot the estimated counts it looks highly significant, which leads me to question my model set-up.