Small primer fixing

Author: vaulot

README.md

@@ -46,7 +46,7 @@ Vaulot D., Geisen S., Mahé F., Bass D. 2020. pr2-primers: an 18S rRNA primer da
 
 ### Versions
 
-1.1 - 2021-05-17
+1.1.0 - 2021-05-17
 * Panel to test individual primer or probes.
 * Add new primers and primer sets.
 

R_scripts/script_update_shiny_files.R

@@ -39,6 +39,7 @@ cat(readChar(file_param, 1e5))
 
 update_pr2 = FALSE
 update_new_primer_sets = FALSE
+update_stats_primer_sets = FALSE
 
 # ===========================================================
 #     Primer database
@@ -116,7 +117,7 @@ export(primer_sets, file = here(file_name), firstActiveRow = 2)
 
 
 # ===========================================================
-#     PR2 database version 4.12.
+#     Update PR2 database - current version 4.12.
 # ===========================================================
 
 if (update_pr2){
@@ -145,9 +146,9 @@ if (update_pr2){
 
 # ===========================================================
 #      Build the file for all primer sets
+# 
+# Note: This is to be done only when new primer sets are computed
 # ===========================================================
-  
-# This is to be done only when new primer sets are computed
 
 if (update_new_primer_sets){
 
@@ -180,8 +181,17 @@ if (update_new_primer_sets){
 
 }
 
+
 # ===========================================================
-#     Update primer sets and create labels
+#     Update primer sets stats
+# 
+# Note: This is to be done only when new primer sets are computed
+# ===========================================================
+
+
+if (update_stats_primer_sets){
+# ===========================================================
+#     1- Update primer sets and create labels
 # ===========================================================
 
 primer_sets <- primer_sets %>%
@@ -214,7 +224,7 @@ primer_sets_labels <- primer_sets %>%
          specificity) 
 
 # ===========================================================
-#     Summarize matches
+#     2 - Summarize matches
 # ===========================================================
 
 pr2_match_final <- readRDS(file=here(str_c("R_paper/output/pr2_match_", gene_selected ,"_mismatches_", max_mismatch, ".rds")))  
@@ -333,4 +343,8 @@ pr2_match_final <- readRDS(file=here(str_c("R_paper/output/pr2_match_", gene_sel
 
   saveRDS(pr2_match_summary_primer_set, file=here(str_c("R_paper/output/pr2_match_", gene_selected ,"_mismatches_", max_mismatch, "_summary.rds")))
   saveRDS(pr2_match_summary_primer_set_sg, file=here(str_c("R_paper/output/pr2_match_", gene_selected ,"_mismatches_", max_mismatch, "_summary_sg.rds")))
-  saveRDS(pr2_match_summary_primer_set_class, file=here(str_c("R_paper/output/pr2_match_", gene_selected ,"_mismatches_", max_mismatch, "_summary_class.rds")))  
+  saveRDS(pr2_match_summary_primer_set_class, file=here(str_c("R_paper/output/pr2_match_", gene_selected ,"_mismatches_", max_mismatch, "_summary_class.rds")))  
+  
+}
+  
+  

data/primer_sets.rds

@@ -3,15 +3,17 @@
 ## Major steps
 
 1. Make sure that the shiny application works on R studio
-1. Can run docker 
 2. Build docker file 
   * from shiny-verse
-  * Install libraries as needed
+  * Install packages as needed (load install_packages.R)
   * Use the shiny-server command
 3. Test docker locally
-4. Upload to Google cloud
-  * Set up neough memory (2 Go for pr2 primers)
-5. Upload to Docker web site
+4. Upload to GitHub, this will automatically force compilation on Google cloud and Docker site.
+
+If the Docker application has not been yet uploaded to Google
+1. Upload to Google cloud
+  * Set up enough memory (2 Go for pr2 primers)
+2. Upload to Docker web site
 
 ## Building docker image