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Hi, I used following code for DESeq2 workflow but found some differences in results
gse # A SummarizedExperiment-tibble abstraction: 3,532,606 × 30 # Features=36047 | Samples=98 dds <- DESeqDataSet(gse, design = ~ batch + sex + status) dds <- DESeq(dds) res <- results(dds) summary(res) out of 35983 with nonzero total read count adjusted p-value < 0.1 LFC > 0 (up) : 188, 0.52% LFC < 0 (down) : 62, 0.17% outliers [1] : 99, 0.28% low counts [2] : 15312, 43% (mean count < 7)
I also performed same SE object with same variables using tidybulk
da <- gse |> test_differential_abundance(~batch + sex + status, method = "DESeq2") da_res <- da %>% pivot_transcript() # to compare results all.equal(rownames(res), da_res$.feature) [1] TRUE sum(da_res$padj < 0.1, na.rm=TRUE) [1] 321 table(base_sig = res$padj < .1, tidy_sig = da_res$padj < .1) tidy_sig base_sig FALSE TRUE FALSE 20119 255 TRUE 224 26
Please guide where it went wrong?
The text was updated successfully, but these errors were encountered:
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Hi, I used following code for DESeq2 workflow but found some differences in results
I also performed same SE object with same variables using tidybulk
Please guide where it went wrong?
The text was updated successfully, but these errors were encountered: