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total=ERROR: could not find sequence of contig '20' mouse genome (mm39) #261

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SimJohal opened this issue Nov 14, 2024 · 1 comment
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@SimJohal
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Hi,

I am trying to use Arriba on some mouse samples and using the appropriate files provided in download_references.sh but I am repeatedly getting the error 'total=ERROR: could not find sequence of contig '20'

I am running STAR piped into Arriba as in the demo script. I am including a blacklist and protein domains

I'd be grateful for any help provided!

@suhrig
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suhrig commented Nov 14, 2024

This error occurs when the BAM file contains alignments on chromosome 20, but the FastA file does not contain a sequence for this chromosome. Did you align this using a STAR index from human by any chance? Mouse only has 19 autosomes.

What command did you use to run download-references.sh?

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