error in treesub java.io.IOException: Cannot run program "/usr/bin/unbuffer" (in directory "/home/..."): error=2, No such file or directory

[JuaninAR]

How do I solve the error:

java.io.IOException: Cannot run program "/usr/bin/unbuffer" (in directory "/home/DARWINION/jacosta/Documents/prueba-ts"): error=2, No such file or directory
at java.lang.ProcessBuilder.start(ProcessBuilder.java:1048)
at treesub.gui.AnnotatorGUI$RunAction$RunWorker.doInBackground(Unknown Source)
at treesub.gui.AnnotatorGUI$RunAction$RunWorker.doInBackground(Unknown Source)
at javax.swing.SwingWorker$1.call(SwingWorker.java:295)
at java.util.concurrent.FutureTask.run(FutureTask.java:266)
at javax.swing.SwingWorker.run(SwingWorker.java:334)
at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1149)
at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:624)
at java.lang.Thread.run(Thread.java:748)
Caused by: java.io.IOException: error=2, No such file or directory
at java.lang.UNIXProcess.forkAndExec(Native Method)
at java.lang.UNIXProcess.(UNIXProcess.java:247)
at java.lang.ProcessImpl.start(ProcessImpl.java:134)
at java.lang.ProcessBuilder.start(ProcessBuilder.java:1029)
... 8 more

[JuaninAR]

attached a screen with the error from treesub

[JuaninAR]

I solve this problem, we have installed the expect dpkg package and treesub run!!!

[virologist]

Hi, JuaninAR

I meet the exact same problem with you. Could you pl give me some clues for what kinds of expect dpkg package(s) should install?

update

Ok, I figure it out.
install the package named “expect”
sudo apt-get install -y expect

Another question for Tamuri
Should we modify the default Raxml parameters in streesub in order to build a more robust tree to interpret the amnio acid substitutions?
the default parameters
RAXML_OPTIONS=-m GTRGAMMA -T 2 -# 10 -p 12345
modify to
RAXML_OPTIONS=-f a -m GTRGAMMAI -p 12345 -N 10000 -T 16

Thanks for JuaninAR && Tamuri