From 728218f43142dc97ee32ba54636298a917ae4407 Mon Sep 17 00:00:00 2001 From: Connor Date: Tue, 3 May 2016 16:54:44 -0700 Subject: [PATCH] Working on training GUI --- batch/trainingGui.fig | Bin 51051 -> 52321 bytes batch/trainingGui.m | 259 +++++++++++++++++++++++- batch/trainingGui2.fig | Bin 49421 -> 0 bytes batch/trainingGui2.m | 209 ------------------- trainingConstants/showSegRuleGUI.m | 241 ++++++++++++++++++++++ trainingConstants/showTrainingData.m | 41 ++++ trainingConstants/updateTrainingImage.m | 117 +++++++++++ 7 files changed, 648 insertions(+), 219 deletions(-) delete mode 100644 batch/trainingGui2.fig delete mode 100644 batch/trainingGui2.m create mode 100644 trainingConstants/showSegRuleGUI.m create mode 100644 trainingConstants/showTrainingData.m create mode 100644 trainingConstants/updateTrainingImage.m diff --git a/batch/trainingGui.fig b/batch/trainingGui.fig index 5c3d71ebb58787644b380eedae1c3cd8025429f3..f4e4ef0a45fa74f3293a9762d397d041af7ecc1e 100644 GIT binary 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b/batch/trainingGui.m @@ -22,9 +22,13 @@ % Edit the above text to modify the response to help trainingGui -% Last Modified by GUIDE v2.5 29-Apr-2016 16:38:45 +% Last Modified by GUIDE v2.5 03-May-2016 16:08:54 % Begin initialization code - DO NOT EDIT + +global settings; + + gui_Singleton = 1; gui_State = struct('gui_Name', mfilename, ... 'gui_Singleton', gui_Singleton, ... @@ -50,11 +54,26 @@ function trainingGui_OpeningFcn(hObject, eventdata, handles, varargin) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) % varargin command line arguments to trainingGui (see VARARGIN) +global settings; handles.output = hObject; set(handles.figure1, 'units', 'normalized', 'position', [0.1 0.1 0.8 0.8]) guidata(hObject, handles); +handles.directory.String = pwd; + +settings.axisFlag = 0; +settings.frameNumber = 1; +settings.loadFiles = []; +settings.loadDirectory = []; +settings.currentData = []; +settings.handles = handles; +settings.oldData = []; +settings.oldFrame = []; +settings.maxData = 10; + +updateUI(handles); + % UIWAIT makes trainingGui wait for user response (see UIRESUME) % uiwait(handles.figure1); @@ -101,32 +120,74 @@ function try_const_Callback(hObject, eventdata, handles) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) +dirname = fixDir(handles.directory.String); +images = dir([dirname,'*c1*.tif']); + +if isempty(images) && ~isempty(dir([dirname,'/raw_im/*c1*.tif'])) + dirname = [dirname, '/raw_im/']; +end -tryDifferentConstants(handles.directory.String, []); +tryDifferentConstants(dirname, []); -% --- Executes on button press in pushbutton5. -function pushbutton5_Callback(hObject, eventdata, handles) -% hObject handle to pushbutton5 (see GCBO) +% --- Executes on button press in next. +function previous_Callback(hObject, eventdata, handles) +% hObject handle to next (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) +global settings; + +settings.frameNumber = settings.frameNumber - 1; +settings.frameNumber = max(settings.frameNumber, 1); + +settings.currentData = load([settings.loadDirectory,settings.loadFiles(settings.frameNumber).name]); +updateUI(handles); -% --- Executes on button press in pushbutton6. + +% --- Executes on button press in previous. function pushbutton6_Callback(hObject, eventdata, handles) -% hObject handle to pushbutton6 (see GCBO) +% hObject handle to previous (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) -% --- Executes on button press in pushbutton7. -function pushbutton7_Callback(hObject, eventdata, handles) -% hObject handle to pushbutton7 (see GCBO) +% --- Executes on button press in undo. +function undo_Callback(hObject, eventdata, handles) +% hObject handle to undo (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) +global settings; + +if numel(settings.oldData) > 0 + settings.currentData = settings.oldData(1); + settings.frameNumber = settings.oldFrame(1); + + if numel(settings.oldData) > 1 + settings.oldData = settings.oldData(2:end); + settings.oldFrame = settings.oldFrame(2:end); + else + settings.oldData = []; + settings.oldFrame = []; + end + + updateUI(handles); + + try + data = settings.currentData; + + save([settings.loadDirectory, settings.loadFiles(settings.frameNumber).name],'-STRUCT','data'); + catch ME + warning(['Could not save undo changes: ', ME.message]); + end +else + errordlg(['Reached undo limit']); +end + + % --- Executes on button press in toggle_segs. @@ -134,6 +195,31 @@ function toggle_segs_Callback(hObject, eventdata, handles) % hObject handle to toggle_segs (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) +global settings; + +settings.axisFlag = 1; +settings.frameNumber = 1; + +%Make backup folder +try + backupFolder = [settings.loadDirectory(1:end-1), '_old/']; + if ~exist(backupFolder, 'dir') + mkdir(backupFolder); + end + + copyfile(settings.loadDirectory, backupFolder) +catch ME + warning(['Could not back up files: ', ME.message]); +end + +segTrainingDir = handles.directory.String; +settings.loadDirectory = [segTrainingDir,filesep,'xy1',filesep,'seg',filesep]; +settings.loadFiles = dir([settings.loadDirectory,'*seg.mat']); +settings.currentData = load([settings.loadDirectory,settings.loadFiles(settings.frameNumber).name]); + + + +updateUI(handles); % --- Executes on button press in del_areas. @@ -141,7 +227,29 @@ function del_areas_Callback(hObject, eventdata, handles) % hObject handle to del_areas (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) +global settings; +if exist([settings.loadDirectory, 'CONST.mat'], 'file') + CONST = load([settings.loadDirectory, 'CONST.mat']); + + for i = 1 : numel(settings.loadFiles) + data = load([settings.loadDirectory,settings.loadFiles(i).name]); + + + + data = killRegions(data, CONST) + + try + save([settings.loadDirectory, settings.loadFiles(i).name],'-STRUCT','data'); + catch ME + warning(['Could not save changes: ', ME.message]); + end + end + + disp('Bad regions removed'); +else + warning(['Plese segment files first']); +end % --- Executes on button press in train_segs. function train_segs_Callback(hObject, eventdata, handles) @@ -238,3 +346,134 @@ function image_folder_ClickedCallback(hObject, eventdata, handles) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) handles.directory.String = uigetdir; + + +% --- Executes on button press in next. +function next_Callback(hObject, eventdata, handles) +% hObject handle to next (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) +global settings; + +settings.frameNumber = settings.frameNumber + 1; +settings.frameNumber = min(settings.frameNumber, numel(settings.loadFiles)); + +settings.currentData = load([settings.loadDirectory,settings.loadFiles(settings.frameNumber).name]); + +updateUI(handles); + + +function updateUI(handles) +global settings; + +if settings.axisFlag > 0 + FLAGS.im_flag = settings.axisFlag; + FLAGS.S_flag = 0; + FLAGS.t_flag = 0; + + showTrainingData (settings.currentData, FLAGS, handles.viewport); + + if numel(handles.viewport.Children) > 0 + set(handles.viewport.Children(1),'ButtonDownFcn',@imageButtonDownFcn); + end +end + +if settings.axisFlag == 1 + handles.tooltip.String = ['Editing segments. Max frame: ', num2str(numel(settings.loadFiles))]; + handles.frameNumber.Visible = 'on'; + handles.frameNumber.String = num2str(settings.frameNumber); +elseif settings.axisFlag == 0 + handles.tooltip.String = ['Editing regions. Max frame: ', num2str(numel(settings.loadFiles))]; + handles.frameNumber.Visible = 'on'; + handles.frameNumber.String = num2str(settings.frameNumber); +else + handles.tooltip.String = ''; + handles.frameNumber.Visible = 'off'; + + if numel(handles.viewport.Children) > 0 + set(handles.viewport.Children(1),'Visible', 'off'); + end +end + + +% numChildren = numel(viewport.Children); +% for i = 1:numChildren +% viewport.Children(i).HitTest = 'off'; +% end + + +function saveData() +global settings + +try + data = settings.currentData; + + save([settings.loadDirectory, settings.loadFiles(settings.frameNumber).name],'-STRUCT','data'); +catch ME + warning(['Could not save changes: ', ME.message]); +end + + +function addUndo() +global settings + +settings.oldData = [settings.currentData, settings.oldData]; +if numel(settings.oldData) > settings.maxData + settings.oldData = settings.oldData(1:settings.maxData) +end + +settings.oldFrame = [settings.frameNumber, settings.oldFrame]; +if numel(settings.oldFrame) > settings.maxData + settings.oldFrame = settings.oldFrame(1:settings.maxData) +end + + +% --- Executes on mouse press over axes background. +function imageButtonDownFcn(hObject, eventdata, handles) +% hObject handle to viewport (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) +global settings; + +if settings.axisFlag > 0 + FLAGS.im_flag = settings.axisFlag; + FLAGS.S_flag = 0; + FLAGS.t_flag = 0; + + addUndo(); + [settings.currentData, list] = updateTrainingImage(settings.currentData, FLAGS, eventdata.IntersectionPoint(1:2)); + saveData(); + + updateUI(settings.handles); +end + + + +function frameNumber_Callback(hObject, eventdata, handles) +% hObject handle to frameNumber (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'String') returns contents of frameNumber as text +% str2double(get(hObject,'String')) returns contents of frameNumber as a double +global settings; + +settings.frameNumber = str2num(handles.frameNumber.String); +settings.frameNumber = max(1, min(settings.frameNumber, numel(settings.loadFiles))); + +settings.currentData = load([settings.loadDirectory,settings.loadFiles(settings.frameNumber).name]); + +updateUI(handles); + + +% --- Executes during object creation, after setting all properties. +function frameNumber_CreateFcn(hObject, eventdata, handles) +% hObject handle to frameNumber (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: edit controls usually have a white background on Windows. +% See ISPC and COMPUTER. +if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor','white'); +end diff --git a/batch/trainingGui2.fig 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Starting from the left, property value pairs are -% applied to the GUI before trainingGui2_OpeningFcn gets called. An -% unrecognized property name or invalid value makes property application -% stop. All inputs are passed to trainingGui2_OpeningFcn via varargin. -% -% *See GUI Options on GUIDE's Tools menu. Choose "GUI allows only one -% instance to run (singleton)". -% -% See also: GUIDE, GUIDATA, GUIHANDLES - -% Edit the above text to modify the response to help trainingGui2 - -% Last Modified by GUIDE v2.5 29-Apr-2016 16:12:12 - -% Begin initialization code - DO NOT EDIT -gui_Singleton = 1; -gui_State = struct('gui_Name', mfilename, ... - 'gui_Singleton', gui_Singleton, ... - 'gui_OpeningFcn', @trainingGui2_OpeningFcn, ... - 'gui_OutputFcn', @trainingGui2_OutputFcn, ... - 'gui_LayoutFcn', [] , ... - 'gui_Callback', []); -if nargin && ischar(varargin{1}) - gui_State.gui_Callback = str2func(varargin{1}); -end - -if nargout - [varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:}); -else - gui_mainfcn(gui_State, varargin{:}); -end -% End initialization code - DO NOT EDIT - -% --- Executes just before trainingGui2 is made visible. -function trainingGui2_OpeningFcn(hObject, eventdata, handles, varargin) -% This function has no output args, see OutputFcn. -% hObject handle to figure -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) -% varargin command line arguments to trainingGui2 (see VARARGIN) - -handles.output = hObject; -set(handles.figure1, 'units', 'normalized', 'position', [0.25 0.2 0.35 0.7]) -guidata(hObject, handles); - -% UIWAIT makes trainingGui2 wait for user response (see UIRESUME) -% uiwait(handles.figure1); - - -% --- Outputs from this function are returned to the command line. -function varargout = trainingGui2_OutputFcn(hObject, eventdata, handles) -% varargout cell array for returning output args (see VARARGOUT); -% hObject handle to figure -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - -% Get default command line output from handles structure -varargout{1} = handles.output; - - - - -% --- Executes on button press in cut_and_seg. -function cut_and_seg_Callback(hObject, eventdata, handles) -% hObject handle to cut_and_seg (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - -mass = handles.metricdata.density * handles.metricdata.volume; -set(handles.mass, 'String', mass); - -% --- Executes on button press in try_const. -function try_const_Callback(hObject, eventdata, handles) -% hObject handle to try_const (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - -initialize_gui(gcbf, handles, true); - - - - - -% --- Executes on button press in pushbutton5. -function pushbutton5_Callback(hObject, eventdata, handles) -% hObject handle to pushbutton5 (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in pushbutton6. -function pushbutton6_Callback(hObject, eventdata, handles) -% hObject handle to pushbutton6 (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in pushbutton7. -function pushbutton7_Callback(hObject, eventdata, handles) -% hObject handle to pushbutton7 (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in toggle_segs. -function toggle_segs_Callback(hObject, eventdata, handles) -% hObject handle to toggle_segs (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in del_areas. -function del_areas_Callback(hObject, eventdata, handles) -% hObject handle to del_areas (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in train_segs. -function train_segs_Callback(hObject, eventdata, handles) -% hObject handle to train_segs (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in toggle_regs. -function toggle_regs_Callback(hObject, eventdata, handles) -% hObject handle to toggle_regs (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in bad_regs. -function bad_regs_Callback(hObject, eventdata, handles) -% hObject handle to bad_regs (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on button press in train_regs. -function train_regs_Callback(hObject, eventdata, handles) -% hObject handle to train_regs (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - - -% --- Executes on selection change in listbox2. -function listbox2_Callback(hObject, eventdata, handles) -% hObject handle to listbox2 (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - -% Hints: contents = cellstr(get(hObject,'String')) returns listbox2 contents as cell array -% contents{get(hObject,'Value')} returns selected item from listbox2 - - -% --- Executes during object creation, after setting all properties. -function listbox2_CreateFcn(hObject, eventdata, handles) -% hObject handle to listbox2 (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles empty - handles not created until after all CreateFcns called - -% Hint: listbox controls usually have a white background on Windows. -% See ISPC and COMPUTER. -if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) - set(hObject,'BackgroundColor','white'); -end - - - -function dirname_Callback(hObject, eventdata, handles) -% hObject handle to dirname (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) - -% Hints: get(hObject,'String') returns contents of dirname as text -% str2double(get(hObject,'String')) returns contents of dirname as a double - - -% --- Executes during object creation, after setting all properties. -function dirname_CreateFcn(hObject, eventdata, handles) -% hObject handle to dirname (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles empty - handles not created until after all CreateFcns called - -% Hint: edit controls usually have a white background on Windows. -% See ISPC and COMPUTER. -if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) - set(hObject,'BackgroundColor','white'); -end - - -% --- Executes on button press in save. -function save_Callback(hObject, eventdata, handles) -% hObject handle to save (see GCBO) -% eventdata reserved - to be defined in a future version of MATLAB -% handles structure with handles and user data (see GUIDATA) diff --git a/trainingConstants/showSegRuleGUI.m b/trainingConstants/showSegRuleGUI.m new file mode 100644 index 0000000..d748e1e --- /dev/null +++ b/trainingConstants/showSegRuleGUI.m @@ -0,0 +1,241 @@ +function showSegRuleGUI( data, FLAGS, figNum, viewport ) +% showSegRule : shows the segmentation for regions and segments +% +% INPUT : +% data : data file with segmentation information. +% FLAGS : +% .im_flag = 1 : segment view , +% 2 : region view, +% 3 : false color, +% 4 : phase image +% .S_flag = segments/regions' scores +% .t_flag = segments/regions' labels +% .Sj_flag = shows disagreeing segments scores (S_flag must be on +% too) +% +% Copyright (C) 2016 Wiggins Lab +% Written by Paul Wiggins & Stella Stylianidou. +% University of Washington, 2016 +% This file is part of SuperSegger. +% +% SuperSegger is free software: you can redistribute it and/or modify +% it under the terms of the GNU General Public License as published by +% the Free Software Foundation, either version 3 of the License, or +% (at your option) any later version. +% +% SuperSegger is distributed in the hope that it will be useful, +% but WITHOUT ANY WARRANTY; without even the implied warranty of +% MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +% GNU General Public License for more details. +% +% You should have received a copy of the GNU General Public License +% along with SuperSegger. If not, see . + +if (~exist('FLAGS','var') || isempty(FLAGS)) || ~isfield( FLAGS, 'im_flag' ) + FLAGS.im_flag=2; +end + +im_flag = FLAGS.im_flag; + +if ~isfield( FLAGS, 'S_flag' ) % shows all segments scores + FLAGS.S_flag = 0; +end + +S_flag = FLAGS.S_flag; + +if ~isfield( FLAGS, 't_flag' ) % labels for segments + FLAGS.t_flag = 0; +end + +t_flag = FLAGS.t_flag; + + +if ~isfield( FLAGS, 'phase' ) % labels for segments + FLAGS.phase = 0 ; +end + +% shows scores for segments/regions that computer/user disagrees +if ~isfield( FLAGS, 'Sj_flag' ) + FLAGS.Sj_flag = 0; +end + +Sj_flag = FLAGS.Sj_flag; + +if ~exist('figNum','var') || isempty(figNum) + figNum = 4; +end + +segs_good = zeros( size( data.phase ) ); +segs_good_fail = segs_good; +segs_bad = segs_good; +segs_bad_fail = segs_good; +segs_Include = segs_good; +num_segs = numel( data.segs.score(:) ); + +sz = size(segs_good); +backer = 0.7*ag(data.phase); + +%viewport.Children = []; +%viewport.Visible = 'on'; + +axes(viewport); + +if im_flag == 1 + + isnan_score = isnan(data.segs.scoreRaw); + data.segs.score(isnan_score) = 1; + + if ~isfield(data.segs, 'Include' ) + data.segs.Include = 0*data.segs.score+1; + end + + segs_Include = ismember( data.segs.segs_label, find(~data.segs.Include)); + + segs_good = ismember( data.segs.segs_label, find((data.segs.score))); + segs_bad = ismember( data.segs.segs_label, find((~data.segs.score))); +% +% segs_good = ismember( data.segs.segs_label, find(and( ~isnan_score, and(data.segs.score,round(data.segs.scoreRaw))))); +% segs_good_fail = ismember( data.segs.segs_label, find(and( ~isnan_score, and(data.segs.score,~round(data.segs.scoreRaw))))); +% segs_bad_fail = ismember( data.segs.segs_label, find(and( ~isnan_score, and(~data.segs.score,round(data.segs.scoreRaw))))); +% segs_bad = ismember( data.segs.segs_label, find(and( ~isnan_score, and(~data.segs.score,~round(data.segs.scoreRaw))))); +% + segsInlcudeag = ag(segs_Include); + segsGoodag = ag(segs_good); + segsGoodFailag = ag(segs_good_fail); + segs3nag = ag(data.segs.segs_3n ); + segsBadag = ag(segs_bad ); + segsBadFailag = ag(segs_bad_fail); + maskBgag = ag(~data.mask_bg); + + if FLAGS.phase + phaseBackag = uint8(ag(data.segs.phaseMagic)); + else + phaseBackag = uint8(ag(~data.mask_cell)); + end + + imshow( uint8(cat(3,... + 0.1*phaseBackag + 0.3*segs3nag +0.6*(segsGoodag + segsGoodFailag), ... + 0.15*phaseBackag + 0.1*segs3nag + 0.4*(segsGoodFailag+segsBadFailag), ... + 0.2*phaseBackag + 0.4*(segsBadag + segsBadFailag))), ... + 'InitialMagnification', 'fit','Border','tight'); + + + flagger = and( data.segs.Include, ~isnan(data.segs.score) ); + scoreRawTmp = data.segs.scoreRaw(flagger); + scoreTmp = data.segs.score(flagger); + [y_good,x_good] = hist(scoreRawTmp(scoreTmp>0),[-40:2:40]); + [y_bad,x_bad] = hist(scoreRawTmp(~scoreTmp),[-40:2:40]); + + props = regionprops( data.segs.segs_label, 'Centroid' ); + num_segs = numel(props); + + if S_flag && (~t_flag) + for ii = 1:num_segs + r = props(ii).Centroid; + tmp_flag = double(round(data.segs.scoreRaw(ii)))-double(data.segs.score(ii)); + if tmp_flag == 0 + if ~Sj_flag + text( r(1), r(2), num2str( data.segs.scoreRaw(ii), 2), 'Color', [0.5,0.5,0.5] ); + end + else + if data.segs.Include(ii) + text( r(1), r(2), num2str( data.segs.scoreRaw(ii), 2), 'Color', 'w' ); + elseif ~Sj_flag + text( r(1), r(2), num2str( data.segs.scoreRaw(ii), 2), 'Color', 'g' ); + end + end + end + end + + if t_flag + for ii = 1:num_segs + + r = props(ii).Centroid; + text( r(1), r(2), num2str( ii ), 'Color', 'w' ); + end + end + + +elseif im_flag == 2 % region view + + if ~isfield(data,'regs') + data = updateRegionFields (data,CONST) + end + num_regs = data.regs.num_regs; + + if isfield(data.regs,'score') + regs_good_agree = 0.3*double(ag(ismember(data.regs.regs_label,find(data.regs.score & round(data.regs.scoreRaw))))); + regs_good_disagree = double(ag(ismember(data.regs.regs_label,find(data.regs.score & ~round(data.regs.scoreRaw))))); + + regs_bad_agree = 0.3*double(ag(ismember(data.regs.regs_label,find(~data.regs.score & ~round(data.regs.scoreRaw))))); + regs_bad_disagree = double(ag(ismember(data.regs.regs_label,find(~data.regs.score & round(data.regs.scoreRaw))))); + + imshow( cat(3, 0.5*backer + 1*uint8(regs_good_agree+regs_good_disagree), ... + 0.5*backer, ... + 0.5*backer + 1*uint8(regs_bad_agree+regs_bad_disagree)) , 'InitialMagnification', 'fit'); + else + % imshow(label2rgb(data.regs.regs_label)) + + imshow( cat(3, 0.8*backer + 1*ag(data.mask_cell), ... + 0.8*backer, ... + 0.8*backer) , 'InitialMagnification', 'fit'); + + + end + + + if S_flag && (~t_flag) + for ii = 1:num_regs + r = data.regs.props(ii).Centroid; + flagger = 1; + if isfield (data.regs,'score') + flagger = logical(data.regs.score(ii)) == round(data.regs.scoreRaw(ii)); + end + if flagger + text( r(1), r(2), num2str( data.regs.scoreRaw(ii), 2), 'Color', 'w' ); + elseif ~Sj_flag + text( r(1), r(2), num2str( data.regs.scoreRaw(ii), 2), 'Color', [0.5,0.5,0.5] ); + end + end + end + + if t_flag + for ii = 1:num_regs + r = data.regs.props(ii).Centroid; + text( r(1), r(2), num2str( ii ), 'Color', 'w' ); + %text( r(1), r(2), num2str( data.regs.props(ii).Orientation ), 'Color', 'w' ); + end + end + +elseif im_flag == 3 % phase image in jet color + + imshow( data.segs.phaseMagic, [], 'InitialMagnification', 'fit' ); + colormap jet; + +elseif im_flag == 4 % phase image + + backer = ag(data.phase); + imshow( cat(3,backer,backer,backer), 'InitialMagnification', 'fit' ); +elseif im_flag == 5 + +cell_mask = data.mask_cell; +segs_3n = data.segs.segs_3n ; +segs_good = data.segs.segs_good; +segs_bad = data.segs.segs_bad ; + +back = double(0.7*ag( data.phase )); +outline = imdilate( cell_mask, strel( 'square',3) ); +outline = ag(outline-cell_mask); + + imshow(uint8(cat(3,back + 0.1*double(outline)+ double(ag(segs_good+segs_3n)),... + back ,... + back + 0.2*double(ag(segs_bad))+ 0.2*double(ag(~cell_mask)-outline)))); + + drawnow; +end + +% if ~all(axis_current == [ 0 1 0 1]) +% axis(axis_current); +% end + +end \ No newline at end of file diff --git a/trainingConstants/showTrainingData.m b/trainingConstants/showTrainingData.m new file mode 100644 index 0000000..9354003 --- /dev/null +++ b/trainingConstants/showTrainingData.m @@ -0,0 +1,41 @@ +function showTrainingData (data, FLAGS, viewport) +% makeTrainingData : user can click on segments or regions to change score +% from 0 to 1 or vice versa. It updates scores, cell mask, good and bad +% segs. +% +% INPUT : +% data : data file with segments to be modified +% FLAGS : im_flag = 1 for segments, 2 for regions. +% INPUT : +% data : data file with modified segments +% touch_list : list with modified segments/regions +% +% Copyright (C) 2016 Wiggins Lab +% Written by Paul Wiggins, Stella Stylianidou. +% University of Washington, 2016 +% This file is part of SuperSegger. +% +% SuperSegger is free software: you can redistribute it and/or modify +% it under the terms of the GNU General Public License as published by +% the Free Software Foundation, either version 3 of the License, or +% (at your option) any later version. +% +% SuperSegger is distributed in the hope that it will be useful, +% but WITHOUT ANY WARRANTY; without even the implied warranty of +% MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +% GNU General Public License for more details. +% +% You should have received a copy of the GNU General Public License +% along with SuperSegger. If not, see . + + +if ~exist('FLAGS','var') || ~isfield(FLAGS,'im_flag') + FLAGS.im_flag = 1; + FLAGS.S_flag = 0; + FLAGS.t_flag = 0; +end + +showSegRuleGUI( data, FLAGS ,1, viewport); + +%disp ('Click on segment/region to modify.'); + diff --git a/trainingConstants/updateTrainingImage.m b/trainingConstants/updateTrainingImage.m new file mode 100644 index 0000000..d61b03e --- /dev/null +++ b/trainingConstants/updateTrainingImage.m @@ -0,0 +1,117 @@ +function [data,touch_list] = updateTrainingImage (data, FLAGS, x) +% makeTrainingData : user can click on segments or regions to change score +% from 0 to 1 or vice versa. It updates scores, cell mask, good and bad +% segs. +% +% INPUT : +% data : data file with segments to be modified +% FLAGS : im_flag = 1 for segments, 2 for regions. +% INPUT : +% data : data file with modified segments +% touch_list : list with modified segments/regions +% +% Copyright (C) 2016 Wiggins Lab +% Written by Paul Wiggins, Stella Stylianidou. +% University of Washington, 2016 +% This file is part of SuperSegger. +% +% SuperSegger is free software: you can redistribute it and/or modify +% it under the terms of the GNU General Public License as published by +% the Free Software Foundation, either version 3 of the License, or +% (at your option) any later version. +% +% SuperSegger is distributed in the hope that it will be useful, +% but WITHOUT ANY WARRANTY; without even the implied warranty of +% MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +% GNU General Public License for more details. +% +% You should have received a copy of the GNU General Public License +% along with SuperSegger. If not, see . + +im_flag = FLAGS.im_flag ; +touch_list = []; +ss = size(data.phase); + +x = round(x); + +if ~isempty(x) + % creates an image of 51 x 51 of gaussian like point + tmp = zeros([51,51]); + tmp(26,26) = 1; + tmp = 8000-double(bwdist(tmp)); + + rmin = max([1,x(2)-25]); + rmax = min([ss(1),x(2)+25]); + + cmin = max([1,x(1)-25]); + cmax = min([ss(2),x(1)+25]); + + rrind = rmin:rmax; + ccind = cmin:cmax; + + pointSize = [numel(rrind),numel(ccind)]; + + + if im_flag == 1 + + segs = data.segs.segs_good(rrind,ccind) + ... + data.segs.segs_bad(rrind,ccind); + segs = segs>0; + tmp = tmp(26-x(2)+rrind,26-x(1)+ccind).*segs ; + + [~,ind] = max( tmp(:) ); + + % indices in point image for max / closest segment + [sub1, sub2] = ind2sub( pointSize, ind ); + + % closest segments id + ii = data.segs.segs_label(rmin+sub1-1,cmin+sub2-1); + + if ii ~=0 + + hold on; + plot( sub2-1+cmin, sub1-1+rmin, 'w.','MarkerSize', 30) + + % xx and yy are the segments coordinates + [xx,yy] = getBB( data.segs.props(ii).BoundingBox ); + + if data.segs.score(ii) % score is 1 + data.segs.score(ii) = 0; % set to 0 + data.segs.segs_good(yy,xx) ... + = double(~~(data.segs.segs_good(yy,xx)... + - double(data.segs.segs_label(yy,xx)==ii))); + data.segs.segs_bad(yy,xx) = ... + double(~~(data.segs.segs_bad(yy,xx)... + +double(data.segs.segs_label(yy,xx)==ii))); + else + data.segs.score(ii) = 1; + data.segs.segs_good(yy,xx) = ... + double(~~(data.segs.segs_good(yy,xx)+... + double(data.segs.segs_label(yy,xx)==ii))); + data.segs.segs_bad(yy,xx) = ... + double(~~(data.segs.segs_bad(yy,xx)-... + double(data.segs.segs_label(yy,xx)==ii))); + + end + + % updates cell mask + data.mask_cell = double((data.mask_bg - data.segs.segs_good - data.segs.segs_3n)>0); + touch_list = [touch_list, ii]; + end + elseif im_flag == 2 + tmp = tmp(26-x(2)+rrind,26-x(1)+ccind).*data.mask_cell(rrind,ccind); + try + [~,ind] = max( tmp(:) ); + catch ME + printError(ME); + end + + [sub1, sub2] = ind2sub( pointSize, ind ); + ii = data.regs.regs_label(sub1-1+rmin,sub2-1+cmin); + plot( sub2-1+cmin, sub1-1+rmin, 'g.' ); + + if ii + data.regs.score(ii) = ~data.regs.score(ii); + end + end +end \ No newline at end of file