From d375454996f568b6abcabb0274c57086f05e03fa Mon Sep 17 00:00:00 2001 From: asterakin Date: Wed, 4 May 2016 18:36:08 -0700 Subject: [PATCH] modify constants gui and loadConstants now loads anything in the settings folder --- batch/modifyConstValuesGUI.fig | Bin 0 -> 64140 bytes batch/modifyConstValuesGUI.m | 643 +++++++++++++++++ batch/superSeggerGui.fig | Bin 47836 -> 42602 bytes batch/superSeggerGui.m | 105 +-- batch/tryDifferentConstants.m | 53 +- license.txt | 674 ++++++++++++++++++ segmentation/superSeggerOpti.m | 6 +- .../{100XEcnn_FULLCONST.mat => 100XEc.mat} | Bin .../{100xPann_FULLCONST.mat => 100xPa.mat} | Bin .../{60XBaynn_FULLCONST.mat => 60XBay.mat} | Bin settings/{60XEcnn_FULLCONST.mat => 60XEc.mat} | Bin .../{60XEcLBnn_FULLCONST.mat => 60XEcLB.mat} | Bin settings/{60XPann_FULLCONST.mat => 60XPa.mat} | Bin settings/getConstantsList.m | 15 + settings/loadConstantsNN.m | 64 +- trainingConstants/newConstantsTraining.m | 5 +- trainingConstants/showSegRule.m | 2 +- viz/showSegDataPhase.m | 12 +- 18 files changed, 1433 insertions(+), 146 deletions(-) create mode 100644 batch/modifyConstValuesGUI.fig create mode 100644 batch/modifyConstValuesGUI.m create mode 100644 license.txt rename settings/{100XEcnn_FULLCONST.mat => 100XEc.mat} (100%) rename settings/{100xPann_FULLCONST.mat => 100xPa.mat} (100%) rename settings/{60XBaynn_FULLCONST.mat => 60XBay.mat} (100%) rename settings/{60XEcnn_FULLCONST.mat => 60XEc.mat} (100%) rename settings/{60XEcLBnn_FULLCONST.mat => 60XEcLB.mat} (100%) rename settings/{60XPann_FULLCONST.mat => 60XPa.mat} (100%) create mode 100644 settings/getConstantsList.m diff --git a/batch/modifyConstValuesGUI.fig b/batch/modifyConstValuesGUI.fig new file mode 100644 index 0000000000000000000000000000000000000000..3ab4629afd7f3b85f42619203ad76dd35d30a3f6 GIT binary patch literal 64140 zcma&Nbx<77_XUcE00Dvpm*6ge;O-8A;10n(I17urySoQ>X9@1^?#?2M%kuL5ect>3 zb)A}?nW~wto|<#}oO@>!MSm)YijjY3VIx-*{mE=@WoyPnu3~HKV(#$Uj_=<&aak@- 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zAxjg}lg9dR{0ZQ`+posIyv-7YJ^0G75c%L3uY%2=ii()*hv4rYVZRjmqjkLBmUtf5 znc^yrtJCBUeo)ix%jL5aC(`+}cr+2JLX5I%$-!0r#pasT;gPL+KM0`fJPTeA0dIr= ziR+%Vq}z`k+XfJaDc5bAdRy?NloOw4&skZ>sL74J=rT^=<@MjS%!wY?c1=RtO`j0d zK?KENBzTwE^L2CaEWJt?v(;GiTp_)rNm*l?R-0bQAkGUECcVp5;YszP`bviYI46!< z7OTV3j2CaBE{b2b1)7L@_~25n_3ed2U>#XHW`td8pkqTgIvCC`$YXmLp1832P+ErA z+pB}YmWn$uJ1X`&X0!8nqjwayx4mQHHGe(hkO(36ZaKHuvwlBS=0Ey*P#*`{=RzLXykh?BT(%U+Im zL5Yo@#+i(xs~ORSZyLo0%s-eIn$&AHIe!#465rLoLGfG_?nxMbMLc~Ulf+s5Ed_Wx z!)|<`UCRYZ6f-*%25sD_kAKd}{aB7q`G|x>Fhjr6FO7p)((;DxO&RazU(9ejBzZQ- zTg6cBF5{%-na4h2B)lr-)N1eUl?S_U{$4U;B@XFA3KH@ZZG%JC;ziZ6ARWGD((s2l zwK)RvmS`WT<{JNSjyQ Usqq%MtOe_AhR# 0 + set(handles.viewport.Children(1),'ButtonDownFcn',@imageButtonDownFcn); +hold on; +end + +% --- Executes on slider movement. +function slider3_Callback(hObject, eventdata, handles) +% hObject handle to slider3 (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider + + +% --- Executes during object creation, after setting all properties. +function slider3_CreateFcn(hObject, eventdata, handles) +% hObject handle to slider3 (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + + +% --- Executes on slider movement. +function magic_thresh_Callback(hObject, eventdata, handles) +% hObject handle to magic_thresh (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider +val = hObject.Value; +hObject.Value = round(val); +handles.magic_val.String = num2str(hObject.Value); + + +% --- Executes during object creation, after setting all properties. +function magic_thresh_CreateFcn(hObject, eventdata, handles) +% hObject handle to magic_thresh (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + + + +% --- Executes on slider movement. +function cut_intensity_Callback(hObject, eventdata, handles) +% hObject handle to cut_intensity (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider +val = hObject.Value; +hObject.Value = round(val); +handles.cut_int_text.String = num2str(handles.cut_intensity.Value); + + + +% --- Executes during object creation, after setting all properties. +function cut_intensity_CreateFcn(hObject, eventdata, handles) +% hObject handle to cut_intensity (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + + +% --- Executes on slider movement. +function mask_th1_Callback(hObject, eventdata, handles) +% hObject handle to mask_th1 (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider + +val = hObject.Value; +hObject.Value = round(val); +handles.mask_th1_text.String = num2str(hObject.Value); + +% --- Executes during object creation, after setting all properties. +function mask_th1_CreateFcn(hObject, eventdata, handles) +% hObject handle to mask_th1 (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + +function update_text_values (handles) + +handles.magic_val.String = num2str(handles.magic_thresh.Value); +handles.magic_rad_text.String = num2str(handles.magic_radius.Value); +handles.cut_int_text.String = num2str(handles.cut_intensity.Value); +handles.mask_th1_text.String = num2str(handles.mask_th1.Value); +handles.mask_th2_text.String = num2str(handles.mask_th2.Value); +handles.max_wid_text.String = num2str(handles.max_wid.Value); + + +% --- Executes on slider movement. +function mask_th2_Callback(hObject, eventdata, handles) +% hObject handle to mask_th2 (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider + +val = hObject.Value; +hObject.Value = round(val); +handles.mask_th2_text.String = num2str(hObject.Value); + + +% --- Executes during object creation, after setting all properties. +function mask_th2_CreateFcn(hObject, eventdata, handles) +% hObject handle to mask_th2 (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + + +% --- Executes on slider movement. +function magic_radius_Callback(hObject, eventdata, handles) +% hObject handle to magic_radius (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider + +val = round(hObject.Value); +hObject.Value =val; +handles.magic_rad_text.String = num2str(hObject.Value); + +% --- Executes during object creation, after setting all properties. +function magic_radius_CreateFcn(hObject, eventdata, handles) +% hObject handle to magic_radius (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + + +% --- Executes on slider movement. +function min_thresh_Callback(hObject, eventdata, handles) +% hObject handle to min_thresh (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider + +val = hObject.Value; +hObject.Value = round(val); +handles.min_thresh_text.String = num2str(hObject.Value); + + +% --- Executes during object creation, after setting all properties. +function min_thresh_CreateFcn(hObject, eventdata, handles) +% hObject handle to min_thresh (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + + + +% --- Executes during object creation, after setting all properties. +function max_thresh_CreateFcn(hObject, eventdata, handles) +% hObject handle to max_thresh (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); +end + + +% --- Executes on slider movement. +function max_wid_Callback(hObject, eventdata, handles) +% hObject handle to max_wid (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider +val = round(hObject.Value); +hObject.Value=val; +handles.max_wid_text.String = num2str(hObject.Value); + +% --- Executes during object creation, after setting all properties. +function max_wid_CreateFcn(hObject, eventdata, handles) +% hObject handle to max_wid (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: slider controls usually have a light gray background. +if isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor',[.9 .9 .9]); + set(hObject,'Max',50,'Min',5) + hObject.Value = get(hObject,'Min'); + handles.max_wid_text.String = num2str(hObject.Value); +end + + + +% --- Executes on slider movement. +function max_thresh_Callback(hObject, eventdata, handles) +% hObject handle to max_thresh (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'Value') returns position of slider +% get(hObject,'Min') and get(hObject,'Max') to determine range of slider +val = round(hObject.Value); +hObject.Value=val; +handles.max_thresh_text.String = num2str(hObject.Value); +guidata(hObject,handles); + + +function initialize_all (handles) +% magic thresh +set(handles.magic_thresh,'Max',30,'Min',0) +set(handles.magic_thresh,'SliderStep',[0.1 1]) + +% magic radius +set(handles.magic_radius,'Max',50,'Min',1) +set(handles.magic_radius,'SliderStep',[0.1 1]) + + +% cut_intensity +set(handles.cut_intensity,'Max',150,'Min',10) +set(handles.cut_intensity,'SliderStep',[0.1 1]) + +% mask_th1 +set(handles.mask_th1,'Max',100,'Min',10) +set(handles.mask_th1,'SliderStep',[0.1 1]) + +% mask_th2 +set(handles.mask_th2,'Max',100,'Min',0) +set(handles.mask_th2,'SliderStep',[0.1 1]) + +% maximum width +set(handles.max_wid,'Max',30,'Min',5) +disp(get(handles.max_wid,'Max')) +set(handles.max_wid,'SliderStep',[0.1 1]) + +getValuesFromConst(handles) + + +% --- Executes on button press in run. +function run_Callback(hObject, eventdata, handles) +% hObject handle to run (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +global settings; +resValue = get(handles.constants_list,'Value'); +res = handles.constants_list.String{resValue}; +CONST = loadConstantsNN (res,0); +CONST.superSeggerOpti.CUT_INT = handles.cut_intensity.Value; +CONST.superSeggerOpti.MAX_WIDTH = handles.max_wid.Value; +CONST.superSeggerOpti.THRESH2 = handles.mask_th2.Value; +CONST.superSeggerOpti.THRESH1 = handles.mask_th1.Value; +CONST.superSeggerOpti.MAGIC_THRESHOLD = handles.magic_thresh.Value; +CONST.superSeggerOpti.MAGIC_RADIUS = handles.magic_radius.Value; +settings.CONST = CONST; + +imageName = handles.directory.String; +if numel(imageName)<3 || ~strcmp(imageName(end-3:end), '.tif') + image_folder_ClickedCallback([],[],handles); +end +phaseIm = imread(handles.directory.String); +settings.data = superSeggerOpti(phaseIm,[],0,CONST); +guidata(hObject, handles); +updateUI(handles); + + + +function max_width_regOpt_Callback(hObject, eventdata, handles) +% hObject handle to max_width_regOpt (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'String') returns contents of max_width_regOpt as text +% str2double(get(hObject,'String')) returns contents of max_width_regOpt as a double + + + + +% --- Executes during object creation, after setting all properties. +function max_width_regOpt_CreateFcn(hObject, eventdata, handles) +% hObject handle to max_width_regOpt (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: edit controls usually have a white background on Windows. +% See ISPC and COMPUTER. +if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor','white'); +end + + +function maxLengRegOpti_Callback(hObject, eventdata, handles) +% hObject handle to maxLengRegOpti (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: get(hObject,'String') returns contents of maxLengRegOpti as text +% str2double(get(hObject,'String')) returns contents of maxLengRegOpti as a double + +% --- Executes during object creation, after setting all properties. +function maxLengRegOpti_CreateFcn(hObject, eventdata, handles) +% hObject handle to maxLengRegOpti (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + +% Hint: edit controls usually have a white background on Windows. +% See ISPC and COMPUTER. +if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor','white'); +end + + + + +% --- Executes on mouse press over axes background. +function imageButtonDownFcn(hObject, eventdata, handles) +% hObject handle to viewport (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) +global settings; +updateUI(settings.handles); +point = round(eventdata.IntersectionPoint(1:2)); +if isempty(settings.data) + errordlg('Press find segments first'); +end +if ~isfield(settings.data,'regs') + settings.data = intMakeRegs( settings.data, settings.CONST, [], [] ); +end + +data = settings.data; +ss = size(data.phase); +tmp = zeros([51,51]); +tmp(26,26) = 1; +tmp = 8000-double(bwdist(tmp)); +rmin = max([1,point(2)-25]); +rmax = min([ss(1),point(2)+25]); +cmin = max([1,point(1)-25]); +cmax = min([ss(2),point(1)+25]); +rrind = rmin:rmax; +ccind = cmin:cmax; +pointSize = [numel(rrind),numel(ccind)]; +tmp = tmp(26-point(2)+rrind,26-point(1)+ccind).*data.mask_cell(rrind,ccind); +[~,ind] = max( tmp(:) ); + +[sub1, sub2] = ind2sub( pointSize, ind ); +ii = data.regs.regs_label(sub1-1+rmin,sub2-1+cmin); +hold on; +plot( sub2-1+cmin, sub1-1+rmin, 'o', 'MarkerFaceColor', 'g' ); + + +if ii ~=0 + settings.handles.width_length_image.String = (['length : ', num2str(data.regs.info(ii,1)), ... + ' max width : ', num2str(data.regs.info(ii,4))]); +else + disp ('empty') +end + + +% -------------------------------------------------------------------- +function help_button_ClickedCallback(hObject, eventdata, handles) +% hObject handle to help_button (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +message = ({'SuperSeggerOpti - Modifying Constants Module' + '------------------------------------------------------------' + ' ' + ' ' + 'Magic Threshold: Intensities below this are set to 0, so that intensity differences within a cell region are minimized.' + ' ' + 'Magic Radius: radius for the contrast enhancing filter (proportional to the width of the cell)' + ' ' + 'Mask thresh1: intensity threshold used for background mask to separate cell clumps and background' + ' ' + 'Mask thresh2: intensity threshold used for background mask to separate regions between cell' + ' ' + 'Max width: Width threshold for the iterative segment refinement.More segments are added to the regions whose short axis is greater than this number. All other masked regions are assumed to be correctly defined as a cell.' + ' ' + 'Find segments : Identifies segments usings the chosen constants and values in the sliders' + ' ' + 'Min length region optimization : Cells with length smaller than this are considered for further optimization' + ' ' + 'Max width region optimization : Cells with width larger than this are considered for further optimization' + ' ' + }); + +msgbox(message,'HELP'); diff --git a/batch/superSeggerGui.fig b/batch/superSeggerGui.fig index ba42adb60b54c6e8cca3550760bc3c2640caa92f..7520e13d9465958bacb2d31e49c4a17f51224ac2 100644 GIT binary patch literal 42602 zcma&N1y>wR*ENg=cMEO_gy0%{a19Q@2X}X8kl^mY6WraM;O_43Iyl4d=DDx$2Yj`< zSGBC_I$d3-YoA@a6hzg(i;7Wkv#?Pqh^jN2TiKd1Q7PLRxtcpT+wuRqCN9gv#Y83U zY-Z$YW=iE?&rhZKFIO<~qN3uWV&~=O;Nj=|M#aI#&O`P8H>C@~{Fh*56|nvt`3SHhwgiFgtN8o4u2fKw0(!Z$%ER-NcL=ay00cWyc{+= zyuh2^Eo2ij3?>q9mmQEE%;b6WsIQrpmJF|{99*J^8Bb?Et5j~v7Jh! z2uNpK^Qg0pO=X8;HR1WDHTBUL688aXDDZL_}(1!^nu!!nlDCv0uJaf{0bZ 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z0QsMibeguN(8EhKFwu(J=^hlGCK2$q{YvE<{jIq$Re>i#(=2MMwcBZ^L?F+h=sh~K z{kDpQV}UD9_ovjH51p`yL;2~yI0z#JI%+9aOl`TT3rWfszN~f(ZKfkRv-nWj4mM6| zM7c9W7fK4<1vjhC?CZ{X3eMaMXCMtt$dycO0BDPR9# zQ!4+2v#MeaN1JH)xFIo*)fuF z37HhOjZ1tAxbgqRV~7^ncWlPf4se;QLsD<5^@24VdJ#K<9Ua9oVMxjQFS(|?o!1RF zzR63v zCWT-3#k%*P);QzKQ&)%Yylc)o>5dyCpfF1%OBANPs|;z-YrJc; 1 - errordlg ('Please select only one constant.') - return -end - -text = ''; - -if handles.ec60.Value; - text = '60XEc'; -elseif handles.ec100.Value; - text = '100XEc'; -elseif handles.pa100.Value; - text = '100XPa'; -elseif handles.pa60.Value; - text = '60XPa'; -elseif handles.eclb60.Value; - text = '60XEcLB'; -elseif handles.bay60.Value; - text = '60XBay'; -end - - -% get values for constants +% load constants parallel = handles.parallel_flag.Value; -if ~strcmp(text,''); - CONST = loadConstantsNN(text, parallel); -elseif isfield(handles,'CONST') && ~isempty(handles.CONST) - CONST = handles.CONST; -else - errordlg ('No constants selected'); - return -end +resValue = get(handles.constants_list,'Value'); +res = handles.constants_list.String{resValue}; +CONST = loadConstantsNN (res,parallel); + % set constants CONST.trackOpti.NEIGHBOR_FLAG = handles.neighbor_flag.Value; @@ -151,7 +132,6 @@ function segment_images_Callback(hObject, eventdata, handles) CONST.trackOpti.MIN_CELL_AGE = str2num(handles.cell_age.String); CONST.trackOpti.REMOVE_STRAY = handles.remove_stray.Value; - linkVal = get(handles.link_list,'Value'); if linkVal == 1 CONST.trackOpti.linkFun = @multiAssignmentFastOnlyOverlap; @@ -176,37 +156,8 @@ function bthai60_Callback(hObject, eventdata, handles) % tries different constants function try_constants_Callback(hObject, eventdata, handles) -resFlags = {}; -if handles.ec60.Value; - resFlags{end+1} = '60XEc'; -end -if handles.ec100.Value; - resFlags{end+1} = '100XEc'; -end -if handles.pa100.Value; - resFlags{end+1} = '100XPa'; -end -if handles.pa60.Value; - resFlags{end+1} = '60XPa'; -end -if handles.bay60.Value; - resFlags{end+1} = '60XBay'; -end -if handles.eclb60.Value; - resFlags{end+1} = '60XEcLB'; -end +tryDifferentConstants(handles.directory.String); -tryDifferentConstants(handles.directory.String, resFlags); - -% loads constants that user selects -function loadConstMine_Callback(hObject, eventdata, handles) -if get(hObject,'Value') -[filename,path] = uigetfile('*.mat', 'Pick a superSegger constants file'); -handles.CONST = load([path,'/',filename]); -else - handles.CONST = []; -end -guidata(hObject, handles) % opens superSeggerViewer @@ -250,8 +201,6 @@ function cell_age_CreateFcn(hObject, eventdata, handles) end - - % --- Executes on selection change in constants_list. function link_list_Callback(hObject, eventdata, handles) % hObject handle to constants_list (see GCBO) @@ -269,6 +218,30 @@ function link_list_CreateFcn(hObject, eventdata, handles) % eventdata reserved - to be defined in a future version of MATLAB % handles empty - handles not created until after all CreateFcns called +% Hint: listbox controls usually have a white background on Windows. +% See ISPC and COMPUTER. +if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) + set(hObject,'BackgroundColor','white'); +end + + + +% --- Executes on selection change in constants_list. +function constants_list_Callback(hObject, eventdata, handles) +% hObject handle to constants_list (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles structure with handles and user data (see GUIDATA) + +% Hints: contents = cellstr(get(hObject,'String')) returns constants_list contents as cell array +% contents{get(hObject,'Value')} returns selected item from constants_list + + +% --- Executes during object creation, after setting all properties. +function constants_list_CreateFcn(hObject, eventdata, handles) +% hObject handle to constants_list (see GCBO) +% eventdata reserved - to be defined in a future version of MATLAB +% handles empty - handles not created until after all CreateFcns called + % Hint: listbox controls usually have a white background on Windows. % See ISPC and COMPUTER. if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) diff --git a/batch/tryDifferentConstants.m b/batch/tryDifferentConstants.m index 3852107..a8a7fc6 100644 --- a/batch/tryDifferentConstants.m +++ b/batch/tryDifferentConstants.m @@ -1,63 +1,66 @@ -function tryDifferentConstants(dirname,resFlags) +function data = tryDifferentConstants(filename,resFlags) % tryDifferentConstants : displays images of cells segmented with % different constants set in resFlags. It only does the initial % segmentation (only the doSeg part) and not the regions decisions, % linking and error resolution that come after. -% Images need to have the right naming convention - if they don't use +% Images need to have the right naming convention - if they don't use % renameImages before this script. % % INPUT : -% dirname : directory with images +% dirname : directory with images or filename of image (must be .tif) % OUTPUT : % data.SegFile: _seg file from segmentation % .res: constants resolution for each seg file % -% Copyright (C) 2016 Wiggins Lab +% Copyright (C) 2016 Wiggins Lab % Written by Stella Stylianidou % University of Washington, 2016 % This file is part of SuperSegger. -% +% % SuperSegger is free software: you can redistribute it and/or modify % it under the terms of the GNU General Public License as published by % the Free Software Foundation, either version 3 of the License, or % (at your option) any later version. -% +% % SuperSegger is distributed in the hope that it will be useful, % but WITHOUT ANY WARRANTY; without even the implied warranty of % MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the % GNU General Public License for more details. -% +% % You should have received a copy of the GNU General Public License % along with SuperSegger. If not, see . % modify this accoding to the constants you want to try -if nargin < 1 || isempty( dirname ) || strcmp(dirname,'.') - dirname = pwd; +if nargin < 1 || isempty( filename ) || strcmp(filename,'.') + filename = pwd; end if ~exist('resFlags','var') || isempty(resFlags) -%resFlags = {'60XEc','60XA','60XEcLB',... - % '60XPa','60XPaM','60XPaM2','60XBthai','100XEc','100XPa'}; -resFlags = {'60XEc','100XEc','60XEcLB','60XBay','60XPa','100XPa'}; + [possibleConstants] = getConstantsList(); + resFlags = {possibleConstants.resFlag}; end - -dirname = fixDir(dirname); -images = dir([dirname,'*c1*.tif']); - - -if isempty (images) - disp('no images found in the directory with c1.tif.Select an image'); - [lastPhaseImage,dirname , ~] = uigetfile('*.tif', 'Pick an image file'); - if lastPhaseImage == 0 - return; +if strcmp(filename(end-3:end), '.tif') + tempImage = imread(filename); +else % it is a folder + filename = fixDir(filename); + images = dir([filename,'*c1*.tif']); + if isempty (images) + disp('no images found in the directory with c1.tif.Select an image'); + [lastPhaseImage,filename , ~] = uigetfile('*.tif', 'Pick an image file'); + if lastPhaseImage == 0 + return; + end + else + lastPhaseImage = images(end).name; end -else - lastPhaseImage = images(end).name; + tempImage = imread([filename,lastPhaseImage]); end -phase = intCropImage (imread([dirname,lastPhaseImage])); +phase = intCropImage (tempImage); + + numFlags = numel(resFlags); numCols = 3; numRows = ceil(numFlags / numCols); diff --git a/license.txt b/license.txt new file mode 100644 index 0000000..94a9ed0 --- /dev/null +++ b/license.txt @@ -0,0 +1,674 @@ + GNU GENERAL PUBLIC LICENSE + Version 3, 29 June 2007 + + Copyright (C) 2007 Free Software Foundation, Inc. + Everyone is permitted to copy and distribute verbatim copies + of this license document, but changing it is not allowed. + + Preamble + + The GNU General Public License is a free, copyleft license for +software and other kinds of works. + + The licenses for most software and other practical works are designed +to take away your freedom to share and change the works. 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If your program is a subroutine library, you +may consider it more useful to permit linking proprietary applications with +the library. If this is what you want to do, use the GNU Lesser General +Public License instead of this License. But first, please read +. diff --git a/segmentation/superSeggerOpti.m b/segmentation/superSeggerOpti.m index 4ca733b..a8a164b 100644 --- a/segmentation/superSeggerOpti.m +++ b/segmentation/superSeggerOpti.m @@ -88,8 +88,6 @@ MAGIC_RADIUS = CONST.superSeggerOpti.MAGIC_RADIUS; MAGIC_THRESHOLD = CONST.superSeggerOpti.MAGIC_THRESHOLD; CUT_INT = CONST.superSeggerOpti.CUT_INT; -MIN_THRESHOLD = CONST.superSeggerOpti.MIN_THRESHOLD; -MEAN_THRESHOLD = CONST.superSeggerOpti.MEAN_THRESHOLD; SMOOTH_WIDTH = CONST.superSeggerOpti.SMOOTH_WIDTH; MAX_WIDTH = CONST.superSeggerOpti.MAX_WIDTH; A = CONST.superSeggerOpti.A; @@ -245,7 +243,7 @@ % Determine the "good" and "bad" segments -[data] = defineGoodSegs(ws,C2phaseThresh,mask_bg,MIN_THRESHOLD, MEAN_THRESHOLD, A,CONST); +[data] = defineGoodSegs(ws,C2phaseThresh,mask_bg, A,CONST); % Calculate and return the final cell mask @@ -265,7 +263,7 @@ end -function [data] = defineGoodSegs(ws,phase_mg,mask_bg,MIN_THRESHOLD,MEAN_THRESHOLD,A,CONST) +function [data] = defineGoodSegs(ws,phase_mg,mask_bg,A,CONST) % defineGoodSegs is a sub function that uses intensity thresholds to % segregate the set of segments produced by the watershed algorithm % into "good" segments (segs_good) which lie along a real cellular diff --git a/settings/100XEcnn_FULLCONST.mat b/settings/100XEc.mat similarity index 100% rename from settings/100XEcnn_FULLCONST.mat rename to settings/100XEc.mat diff --git a/settings/100xPann_FULLCONST.mat b/settings/100xPa.mat similarity index 100% rename from settings/100xPann_FULLCONST.mat rename to settings/100xPa.mat diff --git a/settings/60XBaynn_FULLCONST.mat b/settings/60XBay.mat similarity index 100% rename from settings/60XBaynn_FULLCONST.mat rename to settings/60XBay.mat diff --git a/settings/60XEcnn_FULLCONST.mat b/settings/60XEc.mat similarity index 100% rename from settings/60XEcnn_FULLCONST.mat rename to settings/60XEc.mat diff --git a/settings/60XEcLBnn_FULLCONST.mat b/settings/60XEcLB.mat similarity index 100% rename from settings/60XEcLBnn_FULLCONST.mat rename to settings/60XEcLB.mat diff --git a/settings/60XPann_FULLCONST.mat b/settings/60XPa.mat similarity index 100% rename from settings/60XPann_FULLCONST.mat rename to settings/60XPa.mat diff --git a/settings/getConstantsList.m b/settings/getConstantsList.m new file mode 100644 index 0000000..deae3fe --- /dev/null +++ b/settings/getConstantsList.m @@ -0,0 +1,15 @@ +function [possibleConstants] = getConstantsList() +%GETCONSTANTSLIST gets all constants files from the settings directory + +FulllocationOfFile = mfilename('fullpath'); +fileSepPosition = find(FulllocationOfFile==filesep,1,'last'); +filepath = FulllocationOfFile ( 1 :fileSepPosition-1); +possibleConstants = dir([filepath,filesep,'*.mat']); + +for i = 1 : numel (possibleConstants) + cName = possibleConstants (i).name; + possibleConstants(i).resFlag =cName (1:end-4); +end + +end + diff --git a/settings/loadConstantsNN.m b/settings/loadConstantsNN.m index 8073a16..0fa6c29 100644 --- a/settings/loadConstantsNN.m +++ b/settings/loadConstantsNN.m @@ -53,32 +53,14 @@ % '100XPa': constants for 100X Pseudemonas % res : {'60XEc','100XEc','60XEcLB','60XBay','60XPa','100XPa'} +[possibleConstants] = getConstantsList(); + +% default values for numbers resFlag = []; -if isa(res,'double' ) && res == 60 - disp('loadConstants: 60X'); - resFlag = '60XEc'; -elseif isa(res,'double' ) && res == 100 - disp('loadConstants: 100X'); - resFlag = '100XEc'; -elseif isa(res, 'char' ); - if strcmpi(res,'60XEc') - resFlag = '60XEc'; - elseif strcmpi(res,'100XEc') - disp('loadConstants: 100X Ecoli'); - resFlag = '100XEc'; - elseif strcmpi(res,'60XEcLB') - disp('loadConstants: 60X LB Ecoli'); - resFlag = '60XEcLB'; - elseif strcmpi(res,'60XBay') - disp('loadConstants: 60X Baylyi'); - resFlag = '60XBay'; - elseif strcmpi(res,'60XPa') - disp('loadConstants: 60X Pseudemonas'); - resFlag = '60XPa'; - elseif strcmpi(res,'100XPa') - disp('loadConstants: 100X Pseudemonas'); - resFlag = '100XPa'; - end +if isa(res,'double' ) && res == 60 + res = '60XEc'; +elseif isa(res,'double' ) && res == 100 + res = '100XEc'; end @@ -132,9 +114,9 @@ % pixelsize -if all(ismember('100X',resFlag)) +if all(ismember('100X',res)) CONST.getLocusTracks.PixelSize = 6/60; -elseif all(ismember('60X',resFlag)) +elseif all(ismember('60X',res)) CONST.getLocusTracks.PixelSize = 6/100; else CONST.getLocusTracks.PixelSize = []; @@ -192,35 +174,25 @@ CONST.SR.Ithresh = 2; % threshold intensity in std for including loci in analysis - -% loading the different values for each constant -if strcmp (resFlag,'60XEc') - ConstLoaded = load('60XEcnn_FULLCONST.mat'); -elseif strcmp (resFlag,'100XEc') - ConstLoaded = load('100XEcnn_FULLCONST.mat'); -elseif strcmp (resFlag,'60XEcLB') - ConstLoaded = load('60XEcLBnn_FULLCONST.mat'); -elseif strcmp (resFlag,'60XBay') - ConstLoaded = load('60XBaynn_FULLCONST.mat'); -elseif strcmp (resFlag,'100XPa') - ConstLoaded = load('100xPann_FULLCONST.mat'); -elseif strcmp (resFlag,'60XPa') - ConstLoaded = load('60XPann_FULLCONST.mat'); +indexConst = find(strcmpi({possibleConstants.name},[res,'.mat'])); +if ~isempty(indexConst) + constFilename = possibleConstants(indexConst).name; + ConstLoaded = load (constFilename); + CONST.ResFlag = constFilename(1:end-4); + disp(['loading Constants : ', constFilename]); else error('loadConstants: Constants not loaded : no match found. Aborting.'); end -% different values from each constant +% values that are loaded separatelly for each constant +% you can add here values that you have changed from the default +% and should be loaded from your constants file. CONST.superSeggerOpti = ConstLoaded.superSeggerOpti; CONST.seg = ConstLoaded.seg; CONST.regionOpti.MAX_WIDTH = ConstLoaded.regionOpti.MAX_WIDTH; CONST.regionOpti.MAX_LENGTH = ConstLoaded.regionOpti.MAX_LENGTH ; -CONST.regionScoreFun.names = ConstLoaded.regionScoreFun.names; -CONST.regionScoreFun.fun =ConstLoaded.regionScoreFun.fun; -CONST.regionScoreFun.props = ConstLoaded.regionScoreFun.props; CONST.regionScoreFun = ConstLoaded.regionScoreFun; -CONST.ResFlag = resFlag; CONST.trackOpti.MIN_AREA= ConstLoaded.trackOpti.MIN_AREA; diff --git a/trainingConstants/newConstantsTraining.m b/trainingConstants/newConstantsTraining.m index dcf7fb1..fb32c54 100644 --- a/trainingConstants/newConstantsTraining.m +++ b/trainingConstants/newConstantsTraining.m @@ -36,7 +36,10 @@ function newConstantsTraining(dirname, constname) % resFlags = {'60XEc','60XA','60XEcLB',... % '60XPa','60XPaM','60XPaM2','60XBthai',... % '100XEc','100XPa'}; -resFlags = {'60XEc','100XEc','60XEcLB','60XBay','60XPa','100XPa'} + +% get the list +[possibleConstants] = getConstantsList(); +resFlags = {possibleConstants.resFlag} % creates a printable verison of resFlgas resFlagsPrint = resFlags ; % work on copy diff --git a/trainingConstants/showSegRule.m b/trainingConstants/showSegRule.m index 477db1c..4dfee2d 100644 --- a/trainingConstants/showSegRule.m +++ b/trainingConstants/showSegRule.m @@ -38,7 +38,7 @@ function showSegRule( data, FLAGS, figNum,CONST ) im_flag = FLAGS.im_flag; if ~isfield( FLAGS, 'S_flag' ) % shows all segments scores - FLAGS.S_flag = 0; + FLAGS.S_flag = 1; end S_flag = FLAGS.S_flag; diff --git a/viz/showSegDataPhase.m b/viz/showSegDataPhase.m index 3b74cec..0e490ae 100644 --- a/viz/showSegDataPhase.m +++ b/viz/showSegDataPhase.m @@ -1,4 +1,4 @@ -function showSegDataPhase( data ) +function showSegDataPhase( data, viewport ) % showSegDataPhase draws the outlines for the regions in the data file. % % INPUT : @@ -8,8 +8,14 @@ function showSegDataPhase( data ) % Unviersity of Washington, 2016 % This file is part of SuperSeggerOpti. +if isempty(data) + return; +end + +if exist('viewport','var') && ~isempty(viewport) + axes(viewport); +end -try back = double(ag( data.phase )); segs_good = data.segs.segs_good; segs_bad = data.segs.segs_bad; @@ -22,5 +28,5 @@ function showSegDataPhase( data ) back + 0.4*double(ag(segs_good)) + 0.3*double(ag(segs_bad)),... back + 0.7*double(ag(segs_bad)) + 0.2*double(ag(~cell_mask)-outline) ))); drawnow; -end + end