RNA-seq-data-analysis-workflow

A workflow for analysing RNA-seq data, aimed at new users who want to understand the methods available.

Workshop timings

DAY 1
Start time: 9:00am
Finish time: 1:00pm

DAY 2
Start time: 9:00am
Finish time: 1:00pm

Workshop Schedule

Day 1

Episode	Duration	Approx Time
Welcome and opening	25 mins	9:00–9:25
Environment log in	10 mins	9:25–9:35
Background	10 mins	9:35–9:45
Sequence quality	55 mins	9:45–10:10
BREAK	10 mins	10:10–10:20
Sequence quality		10:20–10:40
Quantifying gene expression	1 hour	10:40–11:10
BREAK	10 mins	11:10–11:20
Quantifying gene expression		11:20–11:50
Exploratory analysis	1 hour	11:50–12:20
BREAK	10 mins	12:20–12:30
Exploratory analysis		12:30–END

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Day 2

Episode	Duration	Approx Time
Identify differentially expressed genes
t-tests and P-values	30 mins	9:00–9:30
Identify DE w limma	50 mins	9:30–10:00
BREAK	10 mins	10:00–10:10
Identify DE w limma		10:10–10:30
Identify DE w DESeq2	30 mins	10:30–11:00
BREAK	15 mins	11:00–11:15
Functional analysis	1 hour	11:15–12:15
BREAK	5 mins	12:15–12:20
General discussion	~30 mins	12:20–END