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TwinC-Manuscript

Prediction and functional interpretation of inter-chromosomal genome architecture from DNA sequence with TwinC

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Introduction

In this repository, we store scripts to reproduce the analyses presented in Jha et al. (2024). In summary, you can retrain the TwinC classification model on the heart's left or right ventricle, as well as the TwinC regression model on the H1ESC cell line. TwinC is a tool for training, inference, and interpretation of trans-3D genome folding in humans. TwinC uses a Convolutional Neural Network model that predicts contact between two trans genomic loci from nucleotide sequences. In the classification setting, the model takes two 100-kbp nucleotide sequences as input and predicts the likelihood of contact between them. In the regression setting, the model takes two 640-kbp nucleotide sequences as input and predicts the contact scores for a 5 x 5 patch at 128-kbp resolution. Additionally, we have provided scripts to reproduce the figures in the manuscript in figure_notebooks directory.

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