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lucacozzuto committed Apr 3, 2024
2 parents 60c6376 + 253f483 commit ed8ecb1
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2 changes: 1 addition & 1 deletion docs/mop_consensus.rst
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Expand Up @@ -7,7 +7,7 @@ MOP_CONSENSUS
.. autosummary::
:toctree: generated

This module takes as input the output from MOP_MOD with all the four worklows. It outputs the consensus of the diferent predictions running the tool `Nanoconsensus <https://github.com/ADelgadoT/NanoConsensus>`__ in parallel on each transcript for each comparison.
This pipeline takes as input the output from MOP_MOD with all the four worklows. It outputs the consensus of the diferent predictions running the tool `Nanoconsensus <https://github.com/ADelgadoT/NanoConsensus>`__ in parallel on each transcript for each comparison.


Input Parameters
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6 changes: 5 additions & 1 deletion docs/mop_mod.rst
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Expand Up @@ -7,7 +7,11 @@ MOP_MOD
.. autosummary::
:toctree: generated

This module takes as input the output from MOP_PREPROCESS: basecalled fast5 reads, together with their respective fastq files and unspliced alignments to the transcriptome . It runs four different RNA detection algorithms (Epinano, Nanopolish, Tombo and Nanocompore) and it outputs the predictions generated by each one of them as individual tab-delimited files.
This pipeline takes as input the output from MOP_PREPROCESS: basecalled fast5 reads, together with their respective fastq files and unspliced alignments to the transcriptome . It runs four different RNA detection algorithms (Epinano, Nanopolish, Tombo and Nanocompore) and it outputs the predictions generated by each one of them as individual tab-delimited files.

.. image:: ../img/flow_mod.png
:width: 600
:alt: mop_mod graph

Input Parameters
======================
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6 changes: 6 additions & 0 deletions docs/mop_preprocess.rst
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Expand Up @@ -126,6 +126,12 @@ The command line options for each tool used in the pipeline are stored within sp

.. literalinclude:: ../mop_preprocess/tool_opts/drna_tool_seqtagger_opt.tsv

The first column indicates the processing step as **basecalling** or **demultiplexing** etc. Some tools such as Guppy can be used for more processing steps. Several pre-compiled tool_opts files are stored within the folder **tool_opts**.

.. note::
Readucks is run after guppy demultiplexing. It refines the demultiplexing generating different fastqs


Model libraries for specific tools
====================
The following folders are available the respective tools. Some models are already pre-installed-
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11 changes: 9 additions & 2 deletions docs/mop_tail.rst
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Expand Up @@ -7,7 +7,11 @@ MOP_TAIL
.. autosummary::
:toctree: generated

This module takes as input the output from MOP_PREPROCESS: basecalled fast5 reads, together with their respective fastq files, alignment and assignment read ID to gene/transcript. It outputs the estimation of poly(A) tail length at read level provided by **Tailfindr**, **Nanopolish** or both. Tailfinr can be run using three modes: standard, for Nano3P-seq protocol with R9 chemistry and Nano3P-seq protocol with R10 chemistry.
This pipeline takes as input the output from MOP_PREPROCESS: basecalled fast5 reads, together with their respective fastq files, alignment and assignment read ID to gene/transcript. It outputs the estimation of poly(A) tail length at read level provided by **Tailfindr**, **Nanopolish** or both. Tailfinr can be run using three modes: standard, for Nano3P-seq protocol with R9 chemistry and Nano3P-seq protocol with R10 chemistry.

.. image:: ../img/flow_tail.png
:width: 600
:alt: mop_tail graph


Input Parameters
Expand Down Expand Up @@ -71,4 +75,7 @@ Several folders are created by the pipeline within the output directory specifie
"0154ce9c-fe6b-4ebc-bbb1-517fdc524207" 24.05 24.24 "YFL044C"
"020cde28-970d-4710-90a5-977e4b4bbc46" 41.27 56.79 "YGL238W"
If both softwares are run, an additional plot which shows the correlation of their results is generated.
If both programs are run, an additional plot that shows the correlation of their results is generated.

.. image:: ../img/mod_corr.png
:width: 400
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