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Ribosome Binding Sites motifs #37

Merged
merged 18 commits into from
Dec 9, 2024
Merged

Ribosome Binding Sites motifs #37

merged 18 commits into from
Dec 9, 2024

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camilogarciabotero
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@camilogarciabotero camilogarciabotero commented Dec 9, 2024

This PR implements a new feature that detects and scores Ribosome Binding Sites (RBS) Motifs given an ORF. It is based on the possible spacers (offset) of different kinds of DNA motifs:

Spacer Range Representative RBS Motif(s)
3-4 bp GGA, GAG, AGG, AGxAG, AGGAG
5-10 bp GGAG, GAGG, GGxGG, AGGAGG
11-12 bp GGA, GAG, AGG, AGxAG, AGGAG
13-15 bp GGA, GAG, AGG, AGGA, AGGAG

Since there is some overlap we implemented the search of motfs in three different windows:

  • Window Checking A:
                 -10    -3  |-> start codon
               ...|......|..ATG...
  • Window Checking B:
           -16        -5    |-> start codon
         ...|..........|....ATG...
  • Window Checking C:
       -20      -11         |-> start codon
       .|........|..........ATG...

And add more motifs and a basic coding values based on the phannotate idea.

This lead to implement a simple RBS struct to store the motif and other information and three methods related to the detection, scoring and a new coding decision rule (_findrbs, orf_rbs_score, ribsome_binding_site_decision_rule).

…in rbs.md; update make.jl to include RBS section
@camilogarciabotero camilogarciabotero merged commit 326b855 into main Dec 9, 2024
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