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Iterations must be a positive integer: 0 at /opt/wtsi-cgp/bin/brass.pl line 83. #117

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DingTin opened this issue Apr 23, 2023 · 2 comments

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@DingTin
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DingTin commented Apr 23, 2023

Sorry when I use brass I met the problem as the title suggested. I'm using the lated version, and I could not found the solution. Here is my script and the results printed.
script: brass.pl -o /data/brass_sv/test/ -t /data/all_bam/1/1_T.aligned.duplicates_marked.recalibrated.bam -n /data/all_bam/1/1_P.aligned.duplicates_marked.recalibrated.bam -d /data/brass_sv/hg38depth.bed -g /data/brass_sv/fa/GRCh38.primary_assembly.genome.fa -vi /data/brass_sv/2bit/viral.genomic.merged.fa.2bit -ct /data/brass_sv/hg38_centelo.txt -gc /data/brass_sv/homo_sapiens_vep_109_GRCh38.tar.gz -cb /data/brass_sv/hg38_cytoband.txt -ss /data/brass_sv/sampstat1/1_T.txt -mi /data/brass_sv/2bit/all_ncbi_bacteria -s human -as hg38 -pr WXS -c 2
results:
Skipping Sanger_CGP_Brass_Implement_input.1 as previously successful
Skipping Sanger_CGP_Brass_Implement_input.2 as previously successful
Iterations must be a positive integer: 0 at /opt/wtsi-cgp/bin/brass.pl line 83.

@davidrajones
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Hi @DingTin

What are the contents of your /data/brass_sv/fa/GRCh38.primary_assembly.genome.fa.fai and /data/brass_sv/hg38_centelo.txt files. Do the naming conventions align?

@Ajburdett
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Closing issue due to lack of response

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