ndx-fscv Extension for NWB

This NWB extension defines data types for Fast-Scan Cyclic Voltammetry (FSCV), a neurochemical recording technique used to study dopamine and other neuromodulator dynamics. It supports storing the applied triangular ramp waveform and the measured electrochemical current.

Installation

You can install the stable version of the extension from PyPI using pip:

pip install ndx-fscv

If you want to install the development version of the extension you can install it directly from the GitHub repository. The following command installs the development version of the extension:

pip install -U git+https://github.com/catalystneuro/ndx-fscv.git

Usage

Writing FSCV Data

Below is a minimal example of how to use the extension to add FSCV data to an NWB file:

from datetime import datetime
import numpy as np
from dateutil.tz import tzutc
from pynwb import NWBFile, NWBHDF5IO
from ndx_fscv import FSCVExcitationSeries, FSCVResponseSeries

# Create NWBFile
nwbfile = NWBFile(
    session_description="FSCV experiment",
    identifier="FSCV123",
    session_start_time=datetime(2024, 1, 1, tzinfo=tzutc()),
)

# Create FSCVExcitationSeries
applied_voltages = np.random.randn(100,)  # example data with 100 time points
excitation_series = FSCVExcitationSeries(
    name="fscv_excitation_series",
    description="The applied FSCV excitation waveform over time.",
    data=applied_voltages,
    rate=25_000.0,
    scan_frequency=10.0,
    sweep_rate=400.0,
    waveform_shape="Triangle",
    unit="volts",
)

# Create device and electrode group
device = nwbfile.create_device(
    name="fscv_device",
    description="A 4-channel FSCV device.",
)
electrode_group = nwbfile.create_electrode_group(
    name="fscv_electrode_group",
    description="The electrode group for the FSCV electrodes.",
    device=device,
    location="brain region",
)

# Add the electrodes to the NWBFile
for _ in range(4):
    nwbfile.add_electrode(group=electrode_group, location="brain region")

# Create an electrode table region referencing the FSCV electrodes
electrodes = nwbfile.create_electrode_table_region(region=[0, 1, 2, 3], description="FSCV electrodes")

# Create FSCVResponseSeries
measured_currents = np.random.randn(100, 4)  # example data with 100 time points and 4 electrodes
response_series = FSCVResponseSeries(
    name="fscv_response_series",
    description="The measured FSCV response currents over time.",
    data=measured_currents,
    rate=25_000.0,
    electrodes=electrodes,
    excitation_series=excitation_series,
    unit="amperes",
)

# Add to NWBFile
nwbfile.add_acquisition(excitation_series)
nwbfile.add_acquisition(response_series)

# Write to file
with NWBHDF5IO("example_fscv.nwb", "w") as io:
    io.write(nwbfile)

Reading FSCV Data

To read FSCV data from an NWB file:

from pynwb import NWBHDF5IO
from ndx_fscv import FSCVExcitationSeries, FSCVResponseSeries

with NWBHDF5IO("example_fscv.nwb", "r") as io:
    nwbfile = io.read()
    excitation = nwbfile.acquisition["fscv_excitation_series"]
    response = nwbfile.acquisition["fscv_response_series"]
    print(excitation.data[:])
    print(response.data[:])

Visualization

ndx-fscv provides built-in plotting utilities for visualizing FSCV data directly from NWB files.

You will need matplotlib to use these plotting functions:

pip install -U matplotlib

The main plotting functions are:

• plot_series: Plots the FSCV excitation (voltage) and response (current) time series for a specified time window.
• plot_cv: Plots the cyclic voltammogram (CV) for one or more scans, overlaid for each electrode.

Example usage:

from ndx_fscv.plot import plot_series, plot_cv
from pynwb import NWBHDF5IO

with NWBHDF5IO("example_fscv.nwb", "r") as io:
    nwbfile = io.read()
    response = nwbfile.acquisition["fscv_response_series"]
    excitation = nwbfile.acquisition["fscv_excitation_series"]

    # Plot the time series data for the first 0.5 seconds
    plot_series(
        response_series=response,
        excitation_series=excitation,
        start_time=0.0,
        stop_time=0.5,
    )
    # Plot the cyclic voltammogram for the first scan
    plot_cv(response_series=response, excitation_series=excitation, start_scan_index=0, num_scans=1)

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This extension was created using ndx-template.