You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hello ONT-team!
I have a questions/request regarding RNA004 and its trimming.
Up until version 0.7.3 it was possible to use the no-trim parameter to disable adapter trimming for RNA. Ever since version 0.7.4/0.8.0 (4a28d58 if I'm not mistaken) this sadly seems to be no longer possible (as in, no-trim still exists, but has no effect).
This is actually a major problem for us, because we use protocols with custom sequence adapters. And with dorados automated trimming we observe significant overtrimming for them.
Previously, that wasn't a big deal as we just didn't trim and dealt with the adapters in downstream analyses. But now this is no longer possible (with newer versions), which leaves us in a bit of a pickle.
This leads me to my question/request:
Is there any other way to let dorado basecall (with newer versions) without the automated trimming?
Or, could you reenable the no-trim option for RNA adapter trimming?
Out of interest, could you elaborate on why this option was deactivate in the first place?
Thank you very much for your time!
Cheerio
~E
The text was updated successfully, but these errors were encountered:
Question/Feature request
Please describe the issue:
Hello ONT-team!
I have a questions/request regarding RNA004 and its trimming.
Up until version 0.7.3 it was possible to use the
no-trim
parameter to disable adapter trimming for RNA. Ever since version 0.7.4/0.8.0 (4a28d58 if I'm not mistaken) this sadly seems to be no longer possible (as in,no-trim
still exists, but has no effect).This is actually a major problem for us, because we use protocols with custom sequence adapters. And with dorados automated trimming we observe significant overtrimming for them.
Previously, that wasn't a big deal as we just didn't trim and dealt with the adapters in downstream analyses. But now this is no longer possible (with newer versions), which leaves us in a bit of a pickle.
This leads me to my question/request:
Is there any other way to let dorado basecall (with newer versions) without the automated trimming?
Or, could you reenable the
no-trim
option for RNA adapter trimming?Out of interest, could you elaborate on why this option was deactivate in the first place?
Thank you very much for your time!
Cheerio
~E
The text was updated successfully, but these errors were encountered: