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Request: Reenabling the no-trim parameter #1248

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e-fuhrmann opened this issue Feb 10, 2025 · 0 comments
Open

Request: Reenabling the no-trim parameter #1248

e-fuhrmann opened this issue Feb 10, 2025 · 0 comments
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enhancement New feature or request trim Issues related to adapter/primer trimming

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@e-fuhrmann
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Question/Feature request

Please describe the issue:

Hello ONT-team!
I have a questions/request regarding RNA004 and its trimming.

Up until version 0.7.3 it was possible to use the no-trim parameter to disable adapter trimming for RNA. Ever since version 0.7.4/0.8.0 (4a28d58 if I'm not mistaken) this sadly seems to be no longer possible (as in, no-trim still exists, but has no effect).
This is actually a major problem for us, because we use protocols with custom sequence adapters. And with dorados automated trimming we observe significant overtrimming for them.
Previously, that wasn't a big deal as we just didn't trim and dealt with the adapters in downstream analyses. But now this is no longer possible (with newer versions), which leaves us in a bit of a pickle.

This leads me to my question/request:
Is there any other way to let dorado basecall (with newer versions) without the automated trimming?
Or, could you reenable the no-trim option for RNA adapter trimming?
Out of interest, could you elaborate on why this option was deactivate in the first place?

Thank you very much for your time!

Cheerio
~E

@HalfPhoton HalfPhoton added enhancement New feature or request trim Issues related to adapter/primer trimming labels Feb 11, 2025
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Labels
enhancement New feature or request trim Issues related to adapter/primer trimming
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