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…aset Add Dengue virus All serotypes dataset
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| ## Unreleased | ||
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| Initial release for Nextclade v3! | ||
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| Read more about Nextclade datasets in the documentation: https://docs.nextstrain.org/projects/nextclade/en/stable/user/datasets.html |
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| # Dengue dataset | ||
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| | Key | Value | | ||
| | :-- | :-- | | ||
| | name | Dengue virus All serotypes | | ||
| | authors | [Nextstrain](https://nextstrain.org) | | ||
| | reference | NC_002640.1 | | ||
| | workflow | https://github.com/nextstrain/dengue/tree/main/nextclade | | ||
| | path | `nextstrain/dengue/all` | | ||
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| ## Scope of this dataset | ||
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| This dataset assigns serotype (e.g. DENV1-DENV4) to dengue samples based on [criteria outlined by the WHO](https://pubmed.ncbi.nlm.nih.gov/26868382/) and tree placement nearest references [NC_001477.1 (DENV1)](https://www.ncbi.nlm.nih.gov/nuccore/NC_001477.1), [NC_001474.2 (DENV2)](https://www.ncbi.nlm.nih.gov/nuccore/NC_001474.2), [NC_001475.2 (DENV3)](https://www.ncbi.nlm.nih.gov/nuccore/NC_001475.2), and [NC_002640.1 (DENV4)](https://www.ncbi.nlm.nih.gov/nuccore/NC_002640.1). | ||
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| ## Features | ||
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| This dataset supports: | ||
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| - Assignment of serotypes | ||
| - Phylogenetic placement | ||
| - Sequence quality control (QC) | ||
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| ## What are Nextclade datasets | ||
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| Read more about Nextclade datasets in the Nextclade documentation: https://docs.nextstrain.org/projects/nextclade/en/stable/user/datasets.html |
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| ##gff-version 3 | ||
| ##sequence-region Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome 1 10649 | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 102 440 . + . codon_start=1;gene=C;gene_name=C; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 441 713 . + . codon_start=1;gene=pr;gene_name=pr; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 441 938 . + . codon_start=1;gene=M;gene_name=M; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 939 2423 . + . codon_start=1;gene=E;gene_name=E; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 2424 3479 . + . codon_start=1;gene=NS1;gene_name=NS1; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 3480 4133 . + . codon_start=1;gene=NS2A;gene_name=NS2A; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 4134 4523 . + . codon_start=1;gene=NS2B;gene_name=NS2B; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 4524 6377 . + . codon_start=1;gene=NS3;gene_name=NS3; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 6378 6758 . + . codon_start=1;gene=NS4A;gene_name=NS4A; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 6759 6827 . + . codon_start=1;gene=2K;gene_name=2K; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 6828 7562 . + . codon_start=1;gene=NS4B;gene_name=NS4B; | ||
| Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome feature gene 7563 10262 . + . codon_start=1;gene=NS5;gene_name=NS5; |
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| { | ||
| "alignmentParams": { | ||
| "penaltyGapOpen": 8, | ||
| "penaltyGapOpenInFrame": 12, | ||
| "penaltyGapOpenOutOfFrame": 14, | ||
| "gapAlignmentSide": "left", | ||
| "minSeedCover": 0.01, | ||
| "minLength": 1000 | ||
| }, | ||
| "attributes": { | ||
| "name": "Dengue virus All serotypes", | ||
| "reference accession": "NC_002640", | ||
| "reference name": "dengue virus type 4" | ||
| }, | ||
| "compatibility": { | ||
| "cli": "3.0.0-alpha.0", | ||
| "web": "3.0.0-alpha.0" | ||
| }, | ||
| "deprecated": false, | ||
| "enabled": true, | ||
| "experimental": true, | ||
| "files": { | ||
| "changelog": "CHANGELOG.md", | ||
| "examples": "sequences.fasta", | ||
| "genomeAnnotation": "genome_annotation.gff3", | ||
| "pathogenJson": "pathogen.json", | ||
| "readme": "README.md", | ||
| "reference": "reference.fasta", | ||
| "treeJson": "tree.json" | ||
| }, | ||
| "meta": { | ||
| "bugs": "https://github.com/nextstrain/nextclade_data/issues", | ||
| "source code": "https://github.com/nextstrain/nextclade_data" | ||
| }, | ||
| "qc": { | ||
| "frameShifts": { | ||
| "enabled": false | ||
| }, | ||
| "missingData": { | ||
| "enabled": false, | ||
| "missingDataThreshold": 2700, | ||
| "scoreBias": 300 | ||
| }, | ||
| "mixedSites": { | ||
| "enabled": false, | ||
| "mixedSitesThreshold": 10 | ||
| }, | ||
| "privateMutations": { | ||
| "cutoff": 24, | ||
| "enabled": false, | ||
| "typical": 8, | ||
| "weightLabeledSubstitutions": 2, | ||
| "weightReversionSubstitutions": 1, | ||
| "weightUnlabeledSubstitutions": 1 | ||
| }, | ||
| "snpClusters": { | ||
| "clusterCutOff": 5, | ||
| "enabled": false, | ||
| "scoreWeight": 50, | ||
| "windowSize": 100 | ||
| }, | ||
| "stopCodons": { | ||
| "enabled": true | ||
| } | ||
| }, | ||
| "schemaVersion": "3.0.0", | ||
| "version": { | ||
| "tag": "unreleased" | ||
| }, | ||
| "defaultCds": "E" | ||
| } |
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| >Reconstructed_root_sequence_of_https_nextstrain_org_dengue/all/genome | ||
| AGTTGTTAGTCTACGTGGACCGACAAGGACAGTTTCGAATCGGAAGCTTGCTTAACGTAGTTCTAACAGTTTTTTTAATTAGAGAGCAGATCTCTGAGATGAACAACCAACGGAAAAAGGCGGGAAAACCGCCTTTCAATATGCTGAAACGCGAGAGAAACCGCGTGTCAACTGTACAACAGTTGGCGAAGAGATTCTCAATAGGATTGCTGTAAGGGCAAGGACCATTAAAATTGGTTATGGCTTTCATAGCATTCCTACGATTCCTAGCCATCCCACCAACAGCAGGGATTTTGAAAAGATGGGGAACATTCAAGAAGTCTAAGGCTATCAAGGTACTGAGAGGATTCAGGAAGGAGATCGGAAGCATGCTGAACATTTTGAACAGGAGAAAAAGAACAGCAATCATTCTCATGATGCTGATTCCAACAGTAATGGCGTTCCATTTGACCACACGAGATGGAGAGCCGCACATGATTGTGAGCAAGCATGAAAGAGGAAAATCCCTTCTGTTTAAGACAGCGGATGGAATCAACATGTGCACCCTCATTGCCATGGATCTGGGTGAGTTGTGTGAGGACACAATCACGTACAAATGCCCCCTTCTCACCGAAACCGAACCAGAAGACATTGACTGCTGGTGCAATCCCACGTCCACATGGGTGACCTATGGGACGTGTACCCAAACTGGAGAACACAGACGAGAGAAAAGATCAGTGGCACTAGCCCCACATGTGGGAATGGGACTGGAAACAAGAACTGAAACATGGATGTCATCAGAAGGAGCTTGGAAACAAGCCCAGAGAGTAGAGACTTGGATTCTGAGACACCCAGGATTCACGATAATGGCAGTATTTCTGGCATACACCATAGGAACAACATATATCCAGAGAGTCGTTATTTTTATCCTACTGATGCTGGTCGCCCCATCCATGACAATGCGATGCGTGGGAGTAGGAAACAGAGACTTTGTGGAAGGAGTTTCAGGAGGAACGTGGGTTGACGTGGTGCTAGAACATGGAAGTTGTGTCACAACCATGGCAAAGAACAAACCAACACTGGACTTTGAACTGATGAAGACAGAAGCCAAACAACCGGCCACACTAAGGAAATTCTGCATTGAAGCTAAAATAACCAACACAACAACAGAATCAAGATGTCCAACACAAGGGGAACCCAATCTAGTAGAGGAGCAGGACCAGAAGTTCGTCTGCAAACAAACGATGGTGGACAGAGGATGGGGCAATGGCTGTGGTTTGTTTGGAAAAGGAGGCATTGTGACATGTGCGAAGTTTACATGCTTGAAGAAAATAGAAGGAAAAGTGGTGCAACCTGAAAACTTAGAATACACCGTGGTCATAACAGTCCACACAGGAGACGAACACGCAGTGGGAAATGACACAACAAAACATGGAATGACGGCCAAGATAACACCCCAGGCTTCCACCGCAGAAGCTAAACTGACAGACTATGGAACTCTCACGCTGGATTGCTCACCAAGAACAGGTCTCGACTTTAATGAGATGGTCTTGTTGAAAATGAAAAACAAAGCATGGCTTGTACACAGACAATGGTTCCTAGACCTACCTCTACCATGGACATCAGGAGCTGACACATAAGAAGCAAATTGGAACCAGAAAGAGATGCTGGTGACATTCAAAAATCCTCATGCAAAGAAACAGGATGTAGTCGTCCTAGGATCCCAAGAAGGAGCCATGCACACAGCACTCACAGGAGCTACAGAAATCCAAACGTCAGGAGGAAACTTAATGTTTGCAGGACACCTGAAGTGCAGACTGAGAATGGACAAACTGAAACTCAAAGGGATGTCATACGCGATGTGCACAGGAAAGTTCAAAATTGAGAAAGAAATGGCAGAAACACAGCATGGAACAATAGTCATTAAGGTTAAGTATGAAGGAGATGGTGCACCATGCAAGATCCCTTTTGAGATAAAGGATGTGAAAAAGAAAAATGTCATTGGGCGACTGATCACAGCCAACCCAATAGTCACAGAGAAAGACAGTCCAGTCAACATTGAAGCAGAACCTCCTTTTGGGGACAGCTACATCGTGATAGGAGTAGGAGAAAGAGCATTGAAACTCAACTGGTTCAAGAAAGGAAGCTCCATTGGGAAAATGTTTGAGGCAACTGCCAGAGGAGCAAAAAGAATGGCCATCTTGGGAGACACAGCCTGGGATTTTGGATCTGTAGGAGGAGTGTTCACATCAGTAGGAAAGGCGGTGCACCAGGTTTTTGGAACTGTCTATGGGGCTATGTTTAGCGGAGTCTCATGGACCATGAAAATCCTAATAGGGGTTCTCATAACATGGATAGGAATGAATTCAAGAAGCACTTCCCTGTCAATGACATGCATAGCAGTAGGAATCGTCACACTGTACCTGGGAGTCATGGTGCAGGCGGACATGGGTTGTGTTGTGAACTGGAAAGGCAAAGAACTGAAATGTGGAAGTGGAATTTTTGTCACTGACAACGTGCACACTTGGACAGAACAATACAAATTCCAACCAGACTCCCCAACAAGACTGGCGTCAGCCATTCCGAAAGCTCATGAGGAGGGAGTCTGTGGAATCCGATCAGCAACAAGACTGGAGAATCTCATGTGGAAGCAAATAACAAATGAATTGAACCACATCCTATGGGAAAATGAAATCAAACTAACTGTAGTGGCAGGAGACATTAAAGGAATCTTGGAGAAAGGGAAAAGAACACTTACACCTCAACCCATTGAGCTGAAATATTCATGGAAAACATGGGGAAAAGCAAAAATACTCACAGCAGAAGTACAAAATAACACCTTCCTCATAGACGGTCCAGACACACCAGAATGCCCTAATGACCAAAGAGCATGGAATTTTCTGGAAGTTGAGGACTATGGATTTGGAATCTTCACGACCAACATATGGCTGAAATTGCGAGAAGAGTACACCGAAGTGTGTGACCACAGGCTAATGTCAGCAGCCATCAAGGACAACAGGGCCGTCCATGCTGACATGGGCTATTGGATAGAAAGCGCAAAGAATGAGACCTGGAAGATAGAGAAAGCATCCTTCATTGAAGTTAAAACCTGCCTCTGGCCAAAATCACACACTCTTTGGAGCAATGGAGTGCTAGAAAGTGAAATGATAATTCCAAAGATTTTTGCTGGACCAATTTCACAGCACAACTACAGACCAGGCTATTACACACAAACAGCAGGACCATGGCACTTAGGCAAATTGGAGATGGACTTTGATTTTTGTGAAGGAACCACAGTTGTAGTGACTGAGGACTGTGGAAATAGAGGCCCATCTTTGAGAACAACCACAGCCTCAGGAAAACTGATAACTGAATGGTGCTGCCGATCTTGCACATTACCTCCCCTAAGATACATAGGAGAGGATGGATGCTGGTATGGGATGGAAATCAGACCCTTGAATGAGAAAGAAGAGAACCTGGTCAAGTCATTGGTCACAGCCGGGCATGGAAAGGTGGACAATTTTTCAATAGGACTCTTGTGCATGGCATTGTTCATGGAAGAGGTGCTGAGAACCAGAGTTGGCAGAAAACACATGATATTAGTAGTAGTGGTTACCTTCGTGACCCTCATCACAGGGAATATGACATGGAGAGACCTGGTAAGAGTACTTATCATGGTGGGAGCCACTATGTCTGATAGAATGGGGGGCGTGACTTACCTAGCTTTAATGGCAGCTTTCAAAATGAGACCAATCTTCGCAGTGGGATTGTTCTTGAGGAAACTGACTTCAAGAGAAATAATACTGATGACAATAGGAATGGCCCTGGTAACATCGTTCCAAATACCAGATGCCATTGTGGAACTGACTGATGGAATAGCATTGGGGATAATGGTGCTTAAAATGGTGACACATTTTGAAAAATACCAGTTGGGAGCGACCATCCTGTCCTTGACGTGCATCAAAACAACTATGACATTGCATAATGCATGGAAAACAATCACCATTATATTGGCAGTAGTTTCTCTTTTCCCATTGTGCCTGACTTCCTCCCTTCAAAAACAGACAGATTGGATACCAATAACATTGACAATCATAGGAGTCAAACCACTACCCATGTTTCTAATAACTTTAACAAAAACCAGCAAAAAAAGAAGTTGGCCTCTCAATGAGGGCATTATGGCTGTTGGAATGGTGAGCATTCTAGCAAGTTCTCTCCTAAAGAATGATGTTCCCATGGCTGGACCATTAGTGGCTGGAGGCTTGCTCATAGCGTGCTACGTGATAACTGGCAGCTCAGCAGACTTGTCACTGGAGAAAGCCGCCGATGTGAAATGGGAAGAAGAGGCAGAGATATCAGGAGCCAGCCCAATCATATTAGTCACAGTAGAAGAAGATGGTACCATGTCAATAAAGGATGAAGAGGAAGAGAACACACTAACCATACTCATAAAAACAGCACTGCTAGTAGTGTCAGGCCTCTTCCCATTGTCAATACCAGTCACAATGGCGGTATGGTACATGTGGCAAATGAAAAAACAAAGATCCGGAGTGCTGTGGGACGTCCCCTCACCCCCAGCAGTGGAAAAAGCTGAACTGGAAGATGGAGTCTATAGAATCAAGCAAAGAGGGATTTTTGGGAAATCCCAAGTTGGAGTTGGAGTATACAAAGAAGGAGTATTCCACACAATGTGGCACGTCACAAGAGGAGCTGTCCTCATGCATAAAGGGAAGAGACTGGAACCATCTTGGGCTGACGTCAAGAAAGACTTGATATCATATGGAGGAGGTTGGAGGCTTGAAGGAAAATGGAAAAAAGGAGAAGAAGTGCAGGTCTTGGCCGTAGAACCTGGGAAAAATCCAAAAAACGTCCAAACAAAACCTGGCCTTTTCAAGACCACTACAGGAGAAATAGGAGCCGTAGCCCTGGACTTCAAACCTGGAACATCAGGATCTCCCATCATTAACAAAAAAGGAAAAGTTGTGGGTCTCTATGGAAATGGAGTGGTTACAAAAAGTGGAGCCTATGTCAGTGCCATAGCGCAAACTGAAAAAATTGAAGAGCCGAATCCAGAGATTGAAGATGACATCTTTAGAAAGAAAAGATTAACCATAATGGACCTACATCCAGGAGCGGGAAAGACAAAAAGATATCTCCCAGCCATAGTCAGAGAGGCCATAAAAAGAAGCCTGAGAACTCTAATCTTGGCTCCCACAAGAGTGGTGGCAGCTGAAATGGAAGAAGCCCTAAGAGGACTCCCAATAAGTTATCAGACCCCAGCCATGAAGGCTGAACACACAGGAAGGGAGATTGTAGACCTAATGTGCCATGCCACTTTCACCATGAGACTGCTGTCACCAATCAGGGTTCCAAATTACAACCTGATAATAATGGATGAAGCACATTTCACAGACCCAGCCAGCATAGCGGCTAGAGGATACATCTCAACCCGAGTGGAAATGGGAGAGGCAGCTGCAATTTTTATGACAGCCACTCCCCCAGGAACGACAGATCCCTTTCCTCAGAGCAACGCCCCAATCAAAGATGAAGAAAGAGAAATTCCTGAACGCTCATGGAATTCAGGATATGAATGGATCACAGATTTTCAAGGGAAGACAGTGTGGTTTGTTCCAAGCATAAAAGCCGGAAATGACATAGCAAACTGCTTGAGAAAAAATGGAAAAAGGGTGATTCAACTGAGTAGGAAAACCTTTGACACAGAATATCCAAAAACAAAAATCAATGACTGGGACTTTGTGGTCACAACAGACATTTCCGAAATGGGAGCCAATTTCAAAGCTGAGAGAGTGATAGACCCAAGGAGATGCCTGAAGCCAGTTATACTGACAGATGGTCCAGAGCGGGTGATTCTGGCAGGACCCATGCCAGTGACCCTAGCAAGTGCTGCCCAAAGAAGAGGGAGAATAGGAAGGAACCCAAAAAAAGAAAATGACCAGTACATTTACATGGGAGAGCCTCTAAAAAATGATGAAGACCATGCTCACTGGACAGAAGCAAAGATGCTCCTTGACAACATCAACACACCAGAAGGGATCATCCCAACCCTGTTTGAACCAGAAAGGGAAAAGACTGAAGCCATAGACGGTGAATACCGCCTGAGAGGGGAAGCAAGGAAAACCTTTGTGGAACTAATGAGAAGAGGAGACCTACCAGTTTGGCTGGCCTACAAAGTAGCTTCAGAAGGCATCAAATACACAGACAGAAGGTGGTGCTTTGATGGAGAAAGGAACAATCAAATTTTGGAGGAGAACATGGAAGTGGAAATCTGGACAAAAGAAGGAGAAAGGAAGAAATTAAGACCTAGATGGTTGGATGCTAGAACTTATTCTGACCCACTGGCACTAAAGGAATTCAAGGAGTTTGCAGCTGGAAGAAAGTCCATAACCCTTGACCTAATAACAGAAATAGGTAGACTGCCAACTTACTTGACCCACAGGGCCAGAAATGCCCTGGACAACTTGGTCATGCTGCACACGACAGAAAAAGGAGGAAGGGCCTACAAACATGCTCTGAATGAACTACCAGAAACCCTGGAAACATTCATGCTCCTAGCTCTGTTGGCTGCGATGACAGGAGGAATCTTTCTGTTCTTGATGTAAGGAAGAGGTATAGGGAAAATGTCCATGGGACTGATCTGCATAATGGCAGCCAGCGGCCTGCTATGGATGGCAGAAATCCAGCCCCATTGGATAGCGGCCTCAATAATACTGGAGTTTTTTCTCATGGTACTGCTTATTCCAGAACCAGAAAAGCAGAGAACTCCCCAGGACAACCAATTGACCTATGTCGTGATAGCCATCCTCACAATGGTTGCAACAATAGCAGCCAATGAAATGGGATTGTTGGAAAAAACAAAGAAAGATCTTGGGTTTGGCCATGTAGCAACTGAAAATACAATTCTGGACGTAGATCTACGTCCAGCATCAGCATGGACACTCTATGCAGTGGCCACAACAATTATCACACCAATGTTGAGACACACCATAGAAAACTCCTCAGCAAATGTGTCCCTGACAGCCATTGCCAACCAGGCAGCGGTCTTGATGGGTCTTGGAAAAGGATGGCCATTATCGAAAATGGACATAGGAGTTCCACTTCTCGCCTTGGGATGCTATTCACAAGTGAACCCAATAACTCTCACAGCAGCACTACTTTTGCTAGTGGCACATTATGCCATAATAGGTCCAGGACTGCAAGCAAAAGCCACTAGAGAAGCTCAGAAAAGGACAGCAGCTGGAATCATGAAGAATCCAACGGTGGATGGGATAACAGTAATAGACCTAGATCCAATACCCTATGATCCAAAATTTGAAAAGCAGTTAGGACAAGTAATGCTCCTGGTCCTTTGTGCAGCACAGCTACTGTTGATGAGAACTACATGGGCTTTGTGTGAAGCCTTAACCCTAGCCACAGGACCAATATCAACACTGTGGGAAGGAAATCCAGGGAAATTCTGGAACACAACGATAGCTGTGTCCATGGCTAACATTTTCAGAGGGAGTTATTTGGCCGGAGCTGGACTTGCTTTTTCTATCATGAAGAATGCAGGAAGCGCAAGGAGAGGAACTGGGACCATAGGAGAAACACTGGGAGAAAAATGGAAAAGACAACTAAACCCATTGGGCAGGAAAGAATTCGAAATATACAAGAAAAGTGGAATTCTAGAAGTGGACAGAACCGAAGCCAAAGAAGGCTTGAAAAGAGGAGAAACAACCCACCATGCCGTGTCGAGAGGCTCCGCAAAACTGAGATGGTTCGTTGAGAGAAACATGGTCAAACCAGAAGGGAAAGTCGTAGACCTTGGTTGTGGAAGAGGAGGCTGGTCATATTACTGTGCGGGACTGAAGAATGTGACAGAAGTGAAAGGATACACAAAAGGAGGACCAGGACATGAAGAACCAATCCCTATGTCTACATATGGATGGAACCTAGTCAAGCTTCAGAGTGGAGTGGATGTGTTCTTCACTCCACCTGAGAAATGTGACACCCTGTTGTGTGACATTGGGGAATCATCACCAAATCCAACAGTAGAAGAAGGAAGAACATTAAGAGTTCTGAAGATGGTGGAACCATGGCTCAACAACAACACTCAATTTTGCATAAAAGTTCTCAACCCCTACATGCCAACAGTCATAGAAAAACTGGAAAAACTACAAAGGAAACATGGAGGAATCCTTGTGAGAAATCCACTCTCACGAAATTCCACCCATGAGATGTACTGGGTGTCCAATGCTTCAGGAAACATTGTGTCTTCAGTGAACATGATATCAAGAATGTTGCTGAACAGATTCACAATGAGACACAGGAAACCCACTTATGAGAAAGACGTGGACCTTGGAGCTGGAACACGAAATGTCGCAATTGAAACAGAAACACCAAACATGGATATCATTGGGAAAAGGATAGAGAAAATAAAAGAAGAGCATAAAACAACATGGCACTATGATCAGGACAATCCATACAAAACATGGGCTTACCATGGAAGCTATGAAGCCAAACCGACAGGATCAGCATCATCCATGGTGAATGGAGTGGTGAGACTGCTGACAAAACCCTGGGACGTGATTCCCATGGTGACACAGATGGCAATGACAGACACAACTCCCTTTGGACAACAGAGAGTTTTTAAAGAGAAAGTGGACACGAGAACACCAAAACCAAAACCAGGCACAAGAAAAGTTATGAAGATCACAGCCGAGTGGCTTTGGAGATTCCTTGGAAGGAAAAAAAAACCCAGAATGTGCACAAGAGAAGAGTTCACAAAAAAAGTTAGAACCAACGCAGCTATGGGCGCCGTGTTCACTGATGAGAACAAATGGAACTCAGCAAGTGAAGCTGTTGAAGACGAACGGTTTTGGGAACTGGTGGACAAAGAAAGGGATCTTCACCAAGAAGGAAAGTGTGAAACGTGTGTCTACAACATGATGGGAAAAAGAGAGAAGAAACTAGGAGAGTTTGGCAAAGCAAAAGGCAGCAGAGCAATATGGTACATGTGGCTGGGAGCACGCTTCCTAGAGTTTGAAGCCCTAGGTTTCTTGAATGAAGACCACTGGTTCTCCAGAGAAAATTCACTGAGTGGAGTGGAAGGAGAAGGACTGCACAAGCTGGGATACATCCTAAGAGACATAAACAAGAAAGAAGGAGGAGCAATGTATGCTGATGACACAGCTGGTTGGGACACAAGAATCACAGAAGATGACCTGCAGAATGAAGAAATGATCACAGACCAAATGGACCCTGAACATAAGAAATTGGCCAAGGCCATTTTCAAACTAACATACCAAAACAAGGTGGTGAGAGTCCAAAGACCAACACCAAAAGGCACGGTGATGGATATCATATCCAGGAGAGACCAAAGAGGCAGTGGACAGGTTGGAACTTATGGTCTGAACACTTTCACCAACATGGAAGCACAACTAATCAGACAAATGGAAGGTGAAGGAGTCTTTACACCAAAAGAATTCTAGACCCCACATCTGCTAGAAAAAAGAGTTCAAGAATGGTTGGAAAAACATGGAGTCGAAAGGTTAAAAAGAATGGCAATCAGTGGAGATGATTGTGTGGTGAAACCACTAGATGACAGGTTCGCCACAGCCCTAACAGCTTTGAATGACATGGGAAAGGTTAGGAAAGACATACCGCAATGGGAACCTTCAAAAGGATGGAATGATTGGCAACAAGTGCCTTTCTGCTCCCACCACTTTCATGAGCTGATCATGAAAGATGGTCGCACGCTAGTAGTTCCATGCAGAAACCAAGATGAACTGATAGGTAGAGCCAGAATCTCTCAGGGAGCTGGATGGAGCTTGAGAGAAACAGCCTGTCTGGGGAAGTCTTACGCCCAAATGTGGAGGCTGATGTACTTCCACAGAAGAGATCTGAGGCTGGCGGCCAATGCCATATGTTCAGCAGTTCCAGTACATTGGGTTCCAACAAGCAGAACAACCTGGTCGATCCATGCCCACCATCAATGGATGACAACAGAAGACATGTTTACAGTCTGGAACAGGGTGTGGATAGAGGACAACCCATGGATGGAAGACAAAACTCCAGTCTAATCTTGGGAAGACATACCATACCTAGGGAAAAGAGAAGACCAGTGGTGTGGATCCCTGATTGGCCTAACATCCAGGGCCACCTGGGCAAAGAACATTCAAACAGCCATAAACCAAGTCAGAAGCCTCATTGGGAATGAGGAGTATATAGATTACATGCCATCAATGAAAAGATTCAGGAATGAGGAGGAGAACGAAGGAGTTCTGTGGTAAACAGCAAAAGAAGTCAGGCATAAAAGAAGTCAGGCCACTAATGCCACGGCTTGAGCAAACCGTGCTGCCTGTAGCTCCACCAAAAAGGGGAGGTGTAAAAAAACCCCGGGAGGCCACAAACCATGGAAGCTGTACGCATGGTGTAGTGGACTAGCGGTTAGAGGAGACCCCTCCCATGACAGACAAAACGCAGCACAAGGGGGCCCAAAGCCAGGAGGAAGCTGTACTCTCGTTGGAAGGACTAGAGGTTAGAGGAGACCCCCCCAAAACAAAAACAGCATATTGACGCTGGGAAAGACCAGAGATCCTGCTGTCTCCTCAGCATCATTCCAGGCACAGAACGCCAGAAAATGGAATGGTGCTGTTGAATCAACAGGTTCT 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