Releases
1.2.0
nf-core/atacseq v1.2.0 - Iron Swan
[1.2.0] - 2020-07-02
Added
#63 - Added multicore support for Trim Galore!
#75 - Include gene annotation versions in multiqc report
#76 - featureCounts coupled to DESeq2
#79 - Parallelize DESeq2
#80 - Added social preview image
#97 - PBC1, PBC2 from pipeline?
#107 - Add options to change MACS2 parameters
nf-core/chipseq#153 - Add plotHeatmap
nf-core/chipseq#159 - expose bwa mem -T parameter
Regenerated screenshots and added collapsible sections for output files in docs/output.md
Update template to tools 1.9
Replace set
with tuple
and file()
with path()
in all processes
Capitalise process names
Parameters:
--bwa_min_score
to set minimum alignment score for BWA MEM
--macs_fdr
to provide FDR threshold for MACS2 peak calling
--macs_pvalue
to provide p-value threshold for MACS2 peak calling
--skip_peak_qc
to skip MACS2 peak QC plot generation
--skip_peak_annotation
to skip annotation of MACS2 and consensus peaks with HOMER
--skip_consensus_peaks
to skip consensus peak generation
--deseq2_vst
to use variance stabilizing transformation (VST) instead of regularized log transformation (rlog) with DESeq2
--publish_dir_mode
to customise method of publishing results to output directory nf-core/tools#585
Fixed
#71 - consensus_peaks.mLb.clN.boolean.intersect.plot.pdf not generated
#73 - macs_annotatePeaks.mLb.clN.summary.txt file is not created
#86 - bug in the plot_homer_annotatepeaks.r script
#102 - Incorrect Group ID assigned by featurecounts_deseq2.r
#110 - updated AWS test GitHub actions
#109 - Specify custom gtf but gene bed is not generated from that gtf?
nf-core/chipseq#118 - Running on with SGE
nf-core/chipseq#132 - BigWig Error: sort: cannot create temporary file in '': Read-only file system
nf-core/chipseq#154 - computeMatrix.val.mat.gz files not zipped
Make executables in bin/
compatible with Python 3
Dependencies
Add bioconductor-biocparallel 1.20.0
Add markdown 3.2.2
Add pigz 2.3.4
Add pygments 2.6.1
Add pymdown-extensions 7.1
Add python 3.7.6
Add r-reshape2 1.4.4
Add r-tidyr 1.1.0
Update ataqv 1.0.0
-> 1.1.1
Update bedtools 2.27.1
-> 2.29.2
Update bioconductor-deseq2 1.20.0
-> 1.26.0
Update bioconductor-vsn 3.46.0
-> 3.54.0
Update deeptools 3.2.1
-> 3.4.3
Update fastqc 0.11.8
-> 0.11.9
Update gawk 4.2.1
-> 5.1.0
Update homer 4.9.1
-> 4.11
Update macs2 2.1.2
-> 2.2.7.1
Update multiqc 1.7
-> 1.8
Update picard 2.19.0
-> 2.23.1
Update pysam 0.15.2
-> 0.15.3
Update r-base 3.4.1
-> 3.6.2
Update r-ggplot2 3.1.0
-> 3.3.2
Update r-lattice 0.20_35
-> 0.20_41
Update r-optparse 1.6.0
-> 1.6.6
Update r-pheatmap 1.0.10
-> 1.0.12
Update r-scales 1.0.0
-> 1.1.1
Update r-upsetr 1.3.3
-> 1.4.0
Update r-xfun 0.3
-> 0.15
Update samtools 1.9
-> 1.10
Update subread 1.6.4
-> 2.0.1
Update trim-galore 0.5.0
-> 0.6.5
Update ucsc-bedgraphtobigwig 377
-> 357
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