Python 3.7
Program by: Tom Resink (Biochemistry student, Imperial College London)
Simple program that can be utilized to predict the properties of a protein from its primary sequence. It is currently being used to aid in the decision of running size exclusion chromatography or ion exchange chromatography on the secretome of Pichia pastoris
How to use:
- Paste each database accession number for UniProt/SwissProt on a new line in ProteinInput.txt
- navigate to the ProteinAnalysis folder in your terminal
- run Main.py
Current features:
- Predict the charge of multiple polypeptides at different pH
- Predict the mass in kDA of multiple polypeptides
- Linked to SwissProt/UniProt databases
- Can take database accession numbers as input
Current problems:
- bar chart of predicted masses stacks when the protein has the same name as others
Sources: "Properties of Amino Acids," in CRC Handbook of Chemistry and Physics, 99th Edition (Internet Version 2018), John R. Rumble, ed., CRC Press/Taylor & Francis, Boca Raton, FL.