ROI Matching

1. From the initial GUI, click on the ROI Matching button to open the ROI Matching GUI.
2. On the ROI Matching GUI, click the Add Files button to choose data files you would like to process. The data files should belong to the same imaging series, i.e. same field of view, but from different sessions/experiments across days, so they are different enough and cannot be concatenated and treated as a single movie.
3. Use Move up / Move down buttons to adjust the order of the added data files, if necessary.
4. Choose whether to use refined ROIs or not. Refined ROIs refers to the '_refined' variables in the data file, generated by the ROI Refinement module. For example, you could run ROI Refinement module to exclude some unwanted ROIs before running ROI Matching.
5. Plot Matching Result generates figures showing matched and non-matched ROIs between each pair of matchings.
6. Overlap Threshold specifies the threshold for determine if one ROI is a subset of another.
7. The Save Settings button allows you to manually save all settings under a specific file name of your choosing. Settings are saved as .mat files. Note that EZcalcium will automatically save the settings into the .mat data file, so manually saving the settings is usually not necessary. The Load Settings button allows you to load any previously saved settings. You can load the automatically generated .mat file (which is the same file that saves ROI Detection and ROI Refinement results) to check the settings used to generate the results.
8. Once all settings are configured, click the Run ROI Matching button.
9. After ROI matching is completed, new '_matched' variables (e.g. F_raw_matched) would be saved into all data files on the Data Files list, containing only ROIs that appeared (matched) across all sessions (data files), with the same order across all data files.