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@ManavalanG ManavalanG commented May 12, 2025

Pull request

NOTE: This is not a typical PR. Instead this PR acts as documentation on the goal as well as the changes made in this branch.

Please add a clear description of what the PR is about:

Goal: Refactor quac v1.8 to use it with bam and vcf files for rat samples created using parabricks.

Changes made (may not be complete):

  • verifybamID and somalier ancestry tasks were turned off.
  • For somalier relatedness, we created sites data for somalier as described here - https://github.com/uab-cgds-worthey/masala_cafe/pull/4. We tested with sites vcf created using AF from 25 to 150, and they all appeared work well at the least to identify sample identity between tumor and normal samples. Hard to comment ATM on reliability of other relatedness predictions.

Please fill in the checklist below and comment as needed:

  • Was code modified? Briefly describe.
  • Was documentation modified? Briefly describe.
  • Is this a bug-fix? Briefly describe.
  • Is this a feature addition? Briefly describe.
  • Did you modify QuaC-Watch config file? If so, did you modify multiqc template
    configs/multiqc_config_template.jinja2 and script src/quac_watch/create_mutliqc_configs.py as necessary?
  • Did you perform system-level testing manually, using ----cli_cluster_config and --snakemake_cluster_config
    options, as described in the documentation? Did it pass
    completely? If not why?
  • Updated Changelog.md file with change logs in recommended format?

Anything else reviewer should know?

@ManavalanG ManavalanG changed the title Running QuaC with rat samples from parabricks pipeline #98 Refactor QuaC to use with rat data from parabricks pipeline May 12, 2025
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2 participants