Differential Expression

Differential expression is used to compare the expression levels of the genes between samples. Read counting of the samples is a method of performing the same. This is usually followied statistical analysis like Log fold change or Chi-square test to interpret and visualise the differentially expressed genes.

In order to sort the BAM files, Samtools was used. Read count was then performed using HTSeq on each developmental tissue sample combination. The differential expression results were then subject to statistical tests and visualisation using DESeq2 library in R.